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We narrowed to 14,841 results for: flag

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Showing: 10961 - 10980 of 14841 results

  2. pSLQ10625

    Plasmid
    #239268
    Purpose
    Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in primary mouse cortical neurons
    Depositor
    Stanley Qi
    Article
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Insert
    NES-dPspCas13b-EGFP-ABI
    Tags
    Flag tag and V5 tag
    Expression
    Mammalian
    Mutation
    H133A and H1058A in PspCas13b
    Promoter
    CAG
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239268-plasmid-map-sequence-id-474742

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  7. pcDNA3.1-GSDMD_1-275

    Plasmid
    #218230
    Purpose
    Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 1-275
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218230-plasmid-map-sequence-id-430565

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  8. nCaMK2rep

    Plasmid
    #239624
    Purpose
    CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.
    Depositor
    F Javier Díez Guerra
    Article
    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029
    Insert
    PSD95.FingR-NES-SynP3-3xmyc (Syn1 Rat, Synthetic)
    Use
    Cre/Lox and Lentiviral
    Tags
    Flag and myc
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    239624-plasmid-map-sequence-id-476245

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  9. nCaMK2rep-S603A

    Plasmid
    #239625
    Purpose
    Inactive CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.
    Depositor
    F Javier Díez Guerra
    Article
    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029
    Insert
    PSD95.FingR-NES-SynP3-3xmyc (Syn1 Rat, Synthetic)
    Use
    Cre/Lox and Lentiviral
    Tags
    Flag and myc
    Expression
    Mammalian
    Mutation
    Changed serine 603 to Alanine
    Promoter
    hSyn
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    239625-plasmid-map-sequence-id-476241

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  10. pMEDS

    Plasmid
    #236568
    Purpose
    Introduces a multiple expression and dual selection (MEDS) system to use alongside CRISPR for applications such as gene editing.
    Depositor
    Shannon Moonah
    Article
    Sharma et al PLoS One. 2025 May 6;20(5):e0321881. doi
    Inserts
    CD/UPRT (negative selection)
    NPT II (positive selection)
    Tags
    3x Myc and FLAG
    Expression
    Mammalian
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    236568-plasmid-map-sequence-id-477092

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  14. pYPQAT58

    Plasmid
    #223430
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223430-plasmid-map-sequence-id-454181

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  15. pYPQAT59

    Plasmid
    #223431
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223431-plasmid-map-sequence-id-453576

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  16. pYPQAT33

    Plasmid
    #223405
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for dicot plants; NGG PAM; SpCas9D10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-ecTadA8e-SpCas9-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223405-plasmid-map-sequence-id-452221

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  17. pYPQAT37

    Plasmid
    #223409
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223409-plasmid-map-sequence-id-453514

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  18. pYPQAT42

    Plasmid
    #223414
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-ZmUbi-gRNA scaffold-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223414-plasmid-map-sequence-id-454172

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  19. pYPQAT21

    Plasmid
    #223393
    Purpose
    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by 2x35s and the sgRNA was driven by AtU3 promoter.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223393-plasmid-map-sequence-id-453501

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  20. pYPQAT09

    Plasmid
    #223381
    Purpose
    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-SpRY-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223381-plasmid-map-sequence-id-453508

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Showing: 10961 - 10980 of 14841 results