We narrowed to 1,804 results for: FAST

1. pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

   Plasmid

   #113898

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Chicken Mermaid S188

   Plasmid

   #53617

   Purpose

   Genetically encoded voltage sensor based on mUKG-mKOk FRET pair

   Depositor

   Vincent Pieribone

   Article

   Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

   Insert

   Chicken Mermaid S188
   

   Expression

   Mammalian

   Mutation

   Gg-VSP contains an R153Q mutation and an amino ac…

   Promoter

   CMV

   Available Since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Chicken ArcLight A173

   Plasmid

   #53615

   Purpose

   Genetically encoded voltage sensor ArcLight with improved kinetics

   Depositor

   Vincent Pieribone

   Article

   Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

   Insert

   Chicken ArcLight-A173
   

   Expression

   Mammalian

   Mutation

   Gg-VSP contains an R153Q mutation and an amino ac…

   Promoter

   CMV

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-ASAP2s

   Plasmid

   #101276

   Purpose

   Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn (human synapsin)

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJM1-PHD2

   Plasmid

   #223552

   Purpose

   Lentivirus transfer plasmid for expression of full length human PHD2

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

   Insert

   PHD2 (EGLN1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AP1muA single guide RNA

   Plasmid

   #230030

   Purpose

   guide RNA for AP1muA tagging at the C-terminus in pX459

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

   Insert

   AP1M1 guide (AP1M1 Human)
   

   Use

   CRISPR

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSems leader-ALFA-mXFP-TpoR

   Plasmid

   #222945

   Purpose

   Expression of TPOR with ALFAtag and monomeric XFP in mammalian cells

   Depositor

   Jacob Piehler

   Article

   Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

   Insert

   Thrombopoietin receptor (26-635) (MPL Human)
   

   Tags

   ALFAtag, Ig k-chain leader sequence, and mXFP

   Expression

   Mammalian

   Mutation

   mXFP: tryptophan 66 to phenylalanine, TPOR: Signa…

   Promoter

   CMV

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKII-ASAP2s

   Plasmid

   #101275

   Purpose

   Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAMKIIa

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. nLuc_AP10_PPVs_P9mS

   Plasmid

   #119302

   Purpose

   Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logic

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split nLuc fused to antiparallel Coiled-coil AP10 connected with P9mS via PPV cleavable linker
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. P9_TEVs_cLuc

    Plasmid

    #119303

    Purpose

    Expresses C-terminus of split luciferase connected to P9 coil via TEVs cleavable linker/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cLuc connected to P9 coil via TEVs cleavable linker
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Chicken ArcLight Q175

    Plasmid

    #53566

    Purpose

    Genetically encoded voltage sensor ArcLight with improved kinetics

    Depositor

    Vincent Pieribone

    Article

    Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

    Insert

    Chicken ArcLight-Q175
    

    Expression

    Mammalian

    Mutation

    Gg-VSP contains an R153Q mutation and an amino ac…

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Chicken ArcLight S174

    Plasmid

    #53567

    Purpose

    Genetically encoded voltage sensor ArcLight with improved kinetics

    Depositor

    Vincent Pieribone

    Article

    Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

    Insert

    Chicken ArcLight-S174
    

    Expression

    Mammalian

    Mutation

    Gg-VSP contains an R153Q mutation and an amino ac…

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET A-pcStar

    Plasmid

    #226339

    Purpose

    Bacterial expression of RSFP pcStar; photoconvertible red fluorescent protein with high brightness, high photoconversion contrast, and early detectable fluorescence

    Depositor

    Pingyong Xu

    Article

    Zhang et al Nano Lett. 2020 Apr 8;20(4):2197-2208. d

    Insert

    pcStar
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSems meGFP-farnesyl

    Plasmid

    #222949

    Purpose

    Expression of C-terminal fragment of HRAS responsible for farnesylation tagged with GFP in mammalian cells

    Depositor

    Jacob Piehler

    Article

    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

    Insert

    C-terminal fragment of HRAS (170-189) (HRAS Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    C-terminal fragment of HRAS (170-189)

    Promoter

    CMV

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. C99m

    Plasmid

    #121003

    Purpose

    MoClo golden gate assembly CD part for codon-optimized mScarlet-I (fast-folding mScarlet variant, codon-optimized for E. coli; see https://www.fpbase.org/protein/mscarlet-i/).

    Depositor

    Richard Murray

    Insert

    mScarlet-I codon optimized
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-CMV-jGCaMP8f

    Plasmid

    #162373

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8f
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    CMV

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-CMV-jGCaMP8s

    Plasmid

    #162371

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    CMV

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-CMV-jGCaMP8m

    Plasmid

    #162372

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    CMV

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. AP1muA-TurboID-polyA-G418 HR

    Plasmid

    #227734

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSFDuet-1(nsp8-nsp7)(nsp12)

    Plasmid

    #165451

    Purpose

    Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

    Depositor

    Marc Delarue

    Article

    Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

    Inserts

    nsp12
    nsp8
    nsp7
    

    Tags

    14Histidine-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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