Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 2,781 results for: PREP

Showing: 1081 - 1100 of 2781 results

Results per page:

Sort results by:

pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD

Plasmid

#225698

Purpose

Lentiviral expression of AID degron tagged H3.3(K27M) and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K27M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mCherry

Expression

Mammalian

Mutation

K27M

Promoter

pEF1A

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SFFV-FLAG-TurboID-mutKIR-SQSTM1

Plasmid

#223719

Purpose

Expresses FLAG-TurboID-mutKIR-SQSTM1

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Tags

TurboID

Expression

Mammalian

Mutation

Mutation 347-DPSTGE-352 to 347-APSAAA-352

Promoter

SFFV

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHR-SFFV-FLAG-TurboID-mutLIR-SQSTM1

Plasmid

#223718

Purpose

Expresses FLAG-TurboID-mutLIR-SQSTM1

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Tags

TurboID

Expression

Mammalian

Mutation

Mutation 335-DDDW-338 to 335-AAAA-338

Promoter

SFFV

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
AG0058 LGA (DIO-mGlp1r-S33W)

Plasmid

#249036

Purpose

Expression of Mutant Mouse Glp1r-S33W, Cre dependent under hSyn promoter

Depositor

Ali Guler

Article

Godschall et al bioRxiv [Preprint]. 2025 Jun 14:2024.12.

Insert

mGlp1r (S33W)

Use

AAV

Tags

FLAG and HA

Mutation

33S to 33W

Promoter

hSyn

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-ZNF207_∆ZnF_-3xFLAG

Plasmid

#231930

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆ZnF_-3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Insert

ZNF207 (ZNF207 Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of ZnF

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-ZNF207_∆Hel_-3xFLAG

Plasmid

#231931

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆Hel_-3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Insert

ZNF207 (ZNF207 Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of Hel

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-ZNF207_∆QPG_-3xFLAG

Plasmid

#231932

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆QPG_-3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Insert

ZNF207 (ZNF207 Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of QPG

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-ZNF207_∆RNA1-2_-3xFLAG

Plasmid

#231933

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of ZNF207_∆RNA1-2_-3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Insert

ZNF207 (ZNF207 Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of RNA1-2

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-ZNF207-ZnF+Hel_3xFLAG

Plasmid

#247230

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-ZnF+Hel_3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Insert

ZNF207-ZnF+Hel (ZNF207 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-ZNF207-ZnF+Hel_K42E_3xFLAG

Plasmid

#247231

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-ZnF+Hel_K42E_3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Insert

ZNF207-ZnF+Hel_K42E (ZNF207 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-ZNF207-ZnF+Hel_K42M_3xFLAG

Plasmid

#247232

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-ZnF+Hel_K42M_3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Insert

ZNF207-ZnF+Hel_K42M (ZNF207 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-ZNF207-ZnF+Hel_K42R_3xFLAG

Plasmid

#247233

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expresion of ZNF207-ZnF+Hel_K42R_3xFLAG

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Behera et al bioRxiv [Preprint]. 2025 Apr 26:2025.04.

Insert

ZNF207-ZnF+Hel_K42R (ZNF207 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAPLe4 PCMTD1 T62 H67 M68

Plasmid

#246506

Purpose

Recombinant protein expression of PCMTD1 T62 H67 M68

Depositor

Steven Clarke

Article

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Residue 312 is Isoleucine in this plasmid but occ…

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
MKC84_HBB(HBA1reg-HBA1)

Plasmid

#232410

Purpose

AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
MKC83_HBB(HBBreg-HBA1)

Plasmid

#232409

Purpose

AAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD allW D262V

Plasmid

#234616

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_allW D262V (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-LCD allW D262N

Plasmid

#234615

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262N with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_allW D262N (HNRNPA1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) bearing familial ALS …

Promoter

T7

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 (Yellowhammer)

Plasmid

#212402

Purpose

Encodes SARS-CoV-2 spike L5F,D614G,Q677H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L5F,D614G,Q677H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L5F,D614G,Q677H

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 F456L (CTT)

Plasmid

#212542

Purpose

Encodes SARS-CoV-2 Spike F456L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike F456L (CTT) (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

F456L

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Spike-deltaC18 FL.1.5.1

Plasmid

#212539

Purpose

Encodes SARS-CoV-2 variant FL1.5.1 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike FL.1.5.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1081 - 1100 of 2781 results

Results per page:

Sort results by: