Addgene

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pMC-1-ccdB-11

Plasmid

#197815

Purpose

Level 0 J1-J11 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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GGE077

Plasmid

#120779

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Coding sequence of fluorescent protein in position 1 (G1_RedStarII)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

G1_RedStarII

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJK220

Plasmid

#71123

Purpose

Expression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

pantothenate kinase

Use

Tags

H6

Expression

Bacterial

Mutation

Arg175 is mutated to a His

Promoter

T7

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pFH37

Plasmid

#126011

Purpose

Level0 Rice OsU6-2 Pol III promoter with transcription Start-G

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Rice OsU6-2 Pol III promoter with transcription Start-G

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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PL06008A2C06 (smedwi2)

Plasmid

#35698

Depositor

Phillip Newmark

Article

Zayas et al Proc Natl Acad Sci U S A. 2005 Dec 20;10

Insert

Smedwi2

Use

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
miR-124-5 promoter GFP

Plasmid

#20792

Depositor

David Bartel

Article

Shkumatava et al Genes Dev. 2009 Feb 15. 23(4):466-81.

Insert

mir-124 (mir124-5 Zebrafish)

Use

Zebrafish expression

Tags

GFP

Expression

Mutation

Promoter

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only
pCON1-ProA.mCherry

Plasmid

#112519

Purpose

ProA driven mCherry expression with synTT

Depositor

Olivia Steele-Mortimer

Article

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

ProA mCherry TT

Use

Tags

Expression

Bacterial

Mutation

Promoter

ProA

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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UbcH10 DN

Plasmid

#8505

Depositor

Joan Ruderman

Article

Townsley et al Proc Natl Acad Sci U S A 1997 Mar 18;94(

Insert

UbcH10 (UBE2C Human)

Use

Tags

Expression

Bacterial

Mutation

C114S, L118S

Promoter

Available since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
pCON1-ProC.mCherry

Plasmid

#112521

Purpose

ProC driven mCherry expression with synTT

Depositor

Olivia Steele-Mortimer

Article

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

ProC mCherry TT

Use

Tags

Expression

Bacterial

Mutation

Promoter

ProC

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCON1-ProA.gfp

Plasmid

#112515

Purpose

ProA driven gfpmut3 expression with synTT

Depositor

Olivia Steele-Mortimer

Article

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

ProA gfpmut3 TT

Use

Tags

Expression

Bacterial

Mutation

Promoter

ProA

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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UbcH10

Plasmid

#8504

Depositor

Joan Ruderman

Article

Townsley et al Proc Natl Acad Sci U S A 1997 Mar 18;94(

Insert

UbcH10 (UBE2C Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
PL06010B1H03 (smedwi1)

Plasmid

#35699

Depositor

Phillip Newmark

Article

Zayas et al Proc Natl Acad Sci U S A. 2005 Dec 20;10

Insert

Smedwi1

Use

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pUC-TalO8 (Blank-CoTrIP)

Plasmid

#178303

Purpose

Blank CoTrIP plasmid

Depositor

Brian Zid

Article

Chen et al Elife. 2022 Sep 15;11. pii: 76965. doi:

Type

Empty backbone

Use

Tags

NA

Expression

Yeast

Mutation

Promoter

NA

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFH39

Plasmid

#126013

Purpose

Level0 Rice OsU3 Pol III promoter with transcription Start-A

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Rice OsU3 Pol III promoter with transcription Start-A

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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nm-mhc-b

Plasmid

#78709

Purpose

Encodes zebrafish nm-mhc-b

Depositor

Sarah Childs

Article

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

nm-mhc-b (myh10 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
pJK227

Plasmid

#71126

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Use

Tags

GyrA intein

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJK226

Plasmid

#71125

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJK136

Plasmid

#71122

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AarA) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJK376

Plasmid

#70235

Purpose

Protein production from a synthetic gene encoding Treponema denticola ATCC 35405 PurE (UniProt Q73PV9_TREDE)

Depositor

T. J. Kappock

Article

Tranchimand et al Biochemistry. 2011 May 31;50(21):4623-37

Insert

phosphoribosylaminoimidazole carboxylase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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PL06010B1E06 (smedwi3)

Plasmid

#35697

Depositor

Phillip Newmark

Article

Zayas et al Proc Natl Acad Sci U S A. 2005 Dec 20;10

Insert

Smedwi3

Use

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only