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pmCellFree_KA2_ZIKV NS4B

Plasmid

#145367

Purpose

Cell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4B

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV NS2B

Plasmid

#145344

Purpose

Cell-free/ mammalian expression vector for Zika virus NS2B protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2B

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV NS4B

Plasmid

#145347

Purpose

Cell-free/ mammalian expression vector for Zika virus NS4B protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4B

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKVC

Plasmid

#145349

Purpose

Cell-free expression vector for Zika virus Capsid protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Capsid

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf3b

Plasmid

#158373

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf3b

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pmCellFree_KA1_ZIKV NS1

Plasmid

#145342

Purpose

Cell-free/mammalian expression vector for Zika virus NS1 protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS1

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G06_ZIKV NS1

Plasmid

#145352

Purpose

Cell-free expression vector for Zika virus NS1 protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS1

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS4A

Plasmid

#145366

Purpose

Cell-free/mammalian expression vector for Zika virus NS4A protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4A

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV PrM

Plasmid

#145340

Purpose

Cell-free/mammalian expression vector for Zika virus PrM protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV PrM

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

April 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCellFree_KA1_ZIKV NS3

Plasmid

#145345

Purpose

Cell-free/ mammalian expression vector for Zika virus NS3 protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS3

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

April 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCellFree_KA1_ZIKV NS4A

Plasmid

#145346

Purpose

Cell-free/mammalian expression vector for Zika virus NS4A protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4A

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

April 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNB813

Plasmid

#60161

Purpose

Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

yEVenus-PEST

Use

Tags

PEST degron

Expression

Yeast

Mutation

Promoter

SIC1pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pNB330

Plasmid

#60147

Purpose

Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Use

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pSuper.Retro.Puro-WDR5 shRNA 2

Plasmid

#59975

Purpose

Knockdown of WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 shRNA

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCellFree_G06_ZIKV NS4B

Plasmid

#145357

Purpose

Cell-free expression vector for Zika virus NS4B protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4B

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pETM33_OXSR1_CCT

Plasmid

#178482

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

OXSR1_OXSR1 (OXSR1 Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSG5-mERRbeta-short

Plasmid

#52188

Purpose

Expresses mouse ERRbeta short form splice variant in mammalian cells

Depositor

Rebecca Riggins

Article

Heckler et al Cell Cycle. 2014 Dec 12:0.

Insert

Estrogen-related receptor beta (Esrrb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEM-7Zf-TYR

Plasmid

#32786

Depositor

Ruth Halaban

Article

Ujvari et al J Biol Chem. 2001 Feb 23;276(8):5924-31.

Insert

Tyrosinase (TYR Human)

Use

In vitro transcription

Tags

Expression

Mutation

I181M

Promoter

T7

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pSuper.Retro.Puro-WDR5 shRNA 4

Plasmid

#59977

Purpose

Knockdown of WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 shRNA

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only
pCellFree_G06_ZIKV NS2A

Plasmid

#145353

Purpose

Cell-free expression vector for Zika virus NS2A protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2A

Use

Tags

mCherry and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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