We narrowed to 1,172 results for: cas12

1. HBT_pcoCASphi_version2

   Plasmid

   #197932

   Purpose

   Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminal

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pcoCasphi-2
   

   Use

   CRISPR

   Tags

   2xNLS-2xFLAG

   Expression

   Plant

   Mutation

   Promoter

   35SPPDK promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZ001 (AacC2c1 locus)

   Plasmid

   #70166

   Purpose

   Expresses AacC2c1 full locus

   Depositor

   Feng Zhang

   Article

   Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

   Insert

   AacC2c1 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC1750

   Plasmid

   #127984

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC1.530

   Plasmid

   #205441

   Purpose

   Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.

   Depositor

   Alistair McCormick

   Article

   Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

   Insert

   RepB gRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p33Fn

   Plasmid

   #158155

   Purpose

   Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.

   Depositor

   Kabin Xie

   Article

   Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Rice codon optimized

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSIBR002

   Plasmid

   #177665

   Purpose

   SIBR Intron 2 (Int2) interrupts the CDS of FnCas12a

   Depositor

   John van der Oost

   Article

   Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC1748

   Plasmid

   #127982

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available since

   Dec. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTol2-HybridBarcode

   Plasmid

   #174037

   Purpose

   For Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sites

   Depositor

   James Gagnon

   Article

   Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

   Insert

   CRISPR target sites
   

   Use

   Tol2 transgenesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSIBR005

   Plasmid

   #177668

   Purpose

   Constitutive expression of FnCas12a

   Depositor

   John van der Oost

   Article

   Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSIBR001

    Plasmid

    #177628

    Purpose

    SIBR Intron 1 (Int1) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSplit-SWITCHER1-TF1-dsRED

    Plasmid

    #213044

    Purpose

    A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

    Depositor

    Petr Bartunek

    Article

    Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

    Insert

    Split-SWITCHER1-TF1-dsRED
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cBH

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSplit-SWITCHER2-TF1-dsRED

    Plasmid

    #213045

    Purpose

    A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

    Depositor

    Petr Bartunek

    Article

    Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

    Insert

    Split-SWITCHER1-TF1-dsRED
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-PlmCasX(T26R/K610R/K808R)-T2A-EGFP

    Plasmid

    #188274

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    PlmCasX(T26R/K610R/K808R)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC1300_pUB10_pcoCASphi_E9t_MCS_version1

    Plasmid

    #197937

    Purpose

    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTarget_PS_VA

    Plasmid

    #186454

    Purpose

    pTarget containing protospacer for FnCas12a targeting

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTarget_PS_VB

    Plasmid

    #186456

    Purpose

    pTarget containing protospacer for AaCas12b targeting

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ230 (LbCpf1)

    Plasmid

    #86210

    Purpose

    LbCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    LbCpf1
    

    Use

    CRISPR; Gateway compatible lbcpf1 entry clone

    Tags

    5' and 3' NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNHEJ-Cas9-recX

    Plasmid

    #158715

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pY016 (pcDNA3.1-hLbCpf1)

    Plasmid

    #69988

    Purpose

    Expresses humanized LbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pY010 (pcDNA3.1-hAsCpf1)

    Plasmid

    #69982

    Purpose

    Expresses humanized AsCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hAsCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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