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Showing: 1081 - 1100 of 9690 results

  1. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196721-plasmid-map-sequence-id-389753

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  3. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid
    #42335
    Purpose
    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.
    Depositor
    Feng Zhang
    Article
    Cong et al Science. 2013 Jan 3.
    Has Service
    Cloning Grade DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    42335-plasmid-map-sequence-id-275689

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  4. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  5. pDG458

    Plasmid
    #100900
    Purpose
    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100900-plasmid-map-sequence-id-457690

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  6. pIVTR(T7A)-Cas9-T2A-mClover3

    Plasmid
    #172293
    Purpose
    In vitro transcription of Cas9-T2A-mClover3 synthetic construct
    Depositor
    Patrik Verstreken
    Insert
    Cas9-T2A-mClover3
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter A-initiating
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172293-plasmid-map-sequence-id-340312

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  7. LV-tetO-EF1a-GFP

    Plasmid
    #187645
    Purpose
    Lentiviral vector expressing GFP from EF1a promoter downstream from tetO sites
    Depositor
    Martin Carroll
    Insert
    GFP
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187645-plasmid-map-sequence-id-367456

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  8. ipUSEPR-sg-Hs-PCNA

    Plasmid
    #188686
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPCNA (PCNA Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188686-plasmid-map-sequence-id-375116

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  9. pY125 (pcDNA3.1-huBfCpf1)

    Plasmid
    #92301
    Purpose
    Expression of humanized BfCpf1
    Depositor
    Feng Zhang
    Insert
    huBfCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92301-plasmid-map-sequence-id-181503

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  10. pY115 (pcDNA3.1-huMlCpf1)

    Plasmid
    #92275
    Purpose
    Expression of humanized MlCpf1
    Depositor
    Feng Zhang
    Insert
    huMlCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92275-plasmid-map-sequence-id-181494

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  11. pY112 (pcDNA3.1-huSaCpf1)

    Plasmid
    #92269
    Purpose
    Expression of humanized SaCpf1
    Depositor
    Feng Zhang
    Insert
    huSaCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92269-plasmid-map-sequence-id-181496

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  12. pTJK644

    Plasmid
    #138012
    Purpose
    SIX1 knockout
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA2 (SIX1 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    None
    Promoter
    U6
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138012-plasmid-map-sequence-id-275045

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  13. pNM220_AAVS1 integration helper

    Plasmid
    #211864
    Purpose
    Cas9 cutting at AAVS1 safe harbor locus
    Depositor
    Iain Cheeseman
    Insert
    AAVS1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    211864-plasmid-map-sequence-id-426338

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  14. pMEL15_MATz

    Plasmid
    #166099
    Purpose
    Expression of gRNA targeting the MAT locus
    Depositor
    Rebecca Burgess
    Insert
    MAT Z1
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166099-plasmid-map-sequence-id-328795

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  15. pTJK635

    Plasmid
    #138009
    Purpose
    SIX1 knockout
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA2 (SIX1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    None
    Promoter
    U6
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138009-plasmid-map-sequence-id-275004

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  16. pTJK643

    Plasmid
    #138011
    Purpose
    SIX1 knockout
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA1 (SIX1 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    None
    Promoter
    U6
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138011-plasmid-map-sequence-id-275015

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  17. pTJK642

    Plasmid
    #138013
    Purpose
    SIX1 CRISPRi
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA13 (SIX1 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    None
    Promoter
    U6
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138013-plasmid-map-sequence-id-275062

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  18. pC0043-PspCas13b crRNA backbone

    Plasmid
    #103854
    Purpose
    For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6-BbsI-BbsI-PspCas13b DR-polyT
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    103854-plasmid-map-sequence-id-350782

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Showing: 1081 - 1100 of 9690 results