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pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90288

Purpose

BB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Use

Tags

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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sankE3_5

Plasmid

#24893

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

sank E3_5

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
PrbC5

Plasmid

#24787

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb

Use

Tags

His

Expression

Bacterial

Mutation

Asp11Gly, Asp83Asn, Val92Ala, Glu119Gly, and Glu1…

Promoter

Available since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
BS.YxiN.Twin1.a1-368.wt

Plasmid

#13528

Depositor

David McKay

Article

Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

Insert

B. subtilis DEAD-box helicase YxiN first two domains

Use

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

deleted amino acid residues 369-479

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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BS.YxiN.Twin1.a404-479.wt

Plasmid

#13529

Depositor

David McKay

Article

Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

Insert

B. subtilis DEAD-box helicase YxiN specific RNA binding domain

Use

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

delete amino acid residues 1-403

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 Y257W

Plasmid

#1303

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y257W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 257 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 Y507W

Plasmid

#1305

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y507W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 507 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pGALSc104b F130W

Plasmid

#1309

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 F130W (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Changed Phe 130 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pGALSc104b Y257W

Plasmid

#1310

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y257W (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Changed Tyr 257 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGALSc104b Y466W

Plasmid

#1311

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y466W (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Changed Tyr 466 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pGALSc104b Y507W

Plasmid

#1312

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y507W (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Changed Tyr 507 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGALSc104b L553W

Plasmid

#1313

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L553W (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Changed Leu 553 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGALSc104b Y819W

Plasmid

#1314

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y819W (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Changed Tyr 819 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGALSc104b L892W

Plasmid

#1315

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L892W (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Changed Leu 892 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pOxyR_gfp

Plasmid

#194154

Purpose

Green Fluorescent Reporter of intracellular hydrogen peroxide

Depositor

Sven Panke

Article

Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

Insert

transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PoxyR, PoxyS

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTP813

Plasmid

#113308

Purpose

human Mre11 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Bhaskara et al Mol Cell. 2007 Mar 9;25(5):647-61. doi:

Insert

Mre11 (MRE11 Human)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-2A-MCS2

Plasmid

#71782

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA3

Plasmid

#26679

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 . 11():63.

Insert

Karyopherin Alpha 3 (KPNA3 Human)

Use

Tags

T7 (MASMTGGQQMG)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA4

Plasmid

#26680

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 . 11():63.

Insert

Karyopherin Alpha 4 (KPNA4 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA5

Plasmid

#26681

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 . 11():63.

Insert

Karyopherin Alpha 5 (KPNA5 Human)

Use

Tags

T7 (MASMTGGQQMG)

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge