We narrowed to 25,057 results for: Nes

1. pCAS9counter

   Plasmid

   #107192

   Purpose

   Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

   Depositor

   Steve Salipante

   Article

   Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

   Inserts

   Cas9
   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   prab17

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMU-ERr

   Plasmid

   #61170

   Purpose

   Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   AtWAK2-mCherry-HDEL
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available Since

   April 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-TaGu-ENT-3xF-ORF

   Plasmid

   #238488

   Purpose

   For production of C-term flag-tagged TaGu EN-tuned ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Expression

   Mutation

   R1109->A

   Promoter

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJMP8835

   Plasmid

   #220902

   Purpose

   MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PG-dCas9-novo

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-Cre.zf1

   Plasmid

   #61391

   Purpose

   mRNA synthesis of Cre recombinase codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   Cre.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   sp6

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFYF1327 EGFP Site#2

   Plasmid

   #47512

   Purpose

   human gRNA expression vector targeting EGFP

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EGFP site 2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe-vHras-zeo

   Plasmid

   #17756

   Depositor

   Susan Lindquist

   Article

   Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

   Insert

   vHras (genomic0 (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   V12D

   Promoter

   Available Since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

8. Cas9 TLS2

   Plasmid

   #196978

   Purpose

   Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Cas9 with TLS2 tag
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pG10-90

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMP11

   Plasmid

   #215559

   Purpose

   pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Inserts

   Cas9
   gRNA targeting pBR322 origin of replication
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23105 and tet

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRIPZ-Wt-PTEN

    Plasmid

    #206982

    Purpose

    Lentiviral vector for expressing doxycyclin-inducible cDNA of wild type PTEN

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on promoter

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKIR1.1

    Plasmid

    #85758

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Plant

    Mutation

    Promoter

    AtRPS5A

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. RelA cFlag pcDNA3

    Plasmid

    #20012

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Insert

    RelA (Rela Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSico

    Plasmid

    #11578

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. punc503GFP

    Plasmid

    #64021

    Purpose

    503-bp promoter of zebrafish unc-45b drives muscle-specific GFP expression

    Depositor

    Peter Currie, Joachim Berger

    Article

    Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

    Insert

    unc-45b: GFP (unc45b Zebrafish)
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    503-bp promoter of zebrafish unc-45b

    Available Since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. p4489 Flag-betaTrCP

    Plasmid

    #10865

    Depositor

    Peter Howley

    Article

    Zhou et al Mol Cell. 2000 Sep . 6(3):751-6.

    Insert

    betaTrCP (BTRC Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cas9 sgRNA vector

    Plasmid

    #68463

    Purpose

    Cas9 sgRNA vector for cloning

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Punc-4::NGL-1::spGFP1-10

    Plasmid

    #65827

    Purpose

    For trans-synaptic labeling

    Depositor

    Kang Shen, Cori Bargmann

    Article

    Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

    Insert

    NLG-1 (nlg-1 Nematode)
    

    Use

    Tags

    spGFP1-10

    Expression

    Worm

    Mutation

    Promoter

    Punc-4

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT3-EF1aH

    Plasmid

    #180149

    Purpose

    This plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.

    Depositor

    Xin Chen

    Article

    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-mKate2-P2A-TurboID-NS

    Plasmid

    #166568

    Purpose

    Multisite Gateway middle entry clone encoding TurboID with mKate2 marker. Parton lab clone IYN

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    mKate2-P2A-TurboID
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDY1243-Fluorescent RADARS

    Plasmid

    #182540

    Purpose

    Express Fluorescent RADARS in bicistronic vector with mCherry as control gene

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

    Insert

    mNeon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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