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pAc5.1A-DmDCP2-E361Q-dsRNAres-V5His6_J

Plasmid

#146689

Purpose

Insect Expression of DmDCP2-E361Q-dsRNAres

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmDCP2-E361Q-dsRNAres (DCP2 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP231-Lenti-TRE3G-Flag-GluN2B(E1479Q)-WPRE-pA

Plasmid

#164796

Purpose

pLenti vector backbone designed to express Flag Tagged-GluN2B(E1479Q) from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

Flag

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP226-Lenti-CMV-Flag-GluN2B-WRPE-pA

Plasmid

#84275

Purpose

pLenti vector backbone designed to express Flag-GluN2B from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

Flag

Promoter

CMV

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEUK035

Plasmid

#180827

Purpose

T7-inducible expression construct to produce SwGdmB (HY78_09235) in E. coli

Depositor

Gregg Beckham

Article

Bleem et al Chem Catalysis, 2022

Insert

SwGdmB

Tags

6X His

Expression

Bacterial

Promoter

T7

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEUK036

Plasmid

#180828

Purpose

T7-inducible expression construct to produce CnGdmA (CNE_RS35785) in E. coli

Depositor

Gregg Beckham

Article

Bleem et al Chem Catalysis, 2022

Insert

CnGdmA

Tags

6X His

Expression

Bacterial

Promoter

T7

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-9-HSP-11

Plasmid

#176882

Purpose

MoClo Level 0 plasmid, contains HSP

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

HSP terminator

Expression

Plant

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_C'4800EC

Plasmid

#176875

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_D4800EC

Plasmid

#176876

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_A5801

Plasmid

#176877

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

35S:Luc+:HSP

Use

Luciferase

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG4E

Plasmid

#176868

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG5

Plasmid

#176869

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_5

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG6E

Plasmid

#176870

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_6E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-1-CmYLCV-6

Plasmid

#176872

Purpose

MoClo Level 0 plasmid, contains CmYLCV-6 promoter

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

CmYLCV

Expression

Plant

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_A4800EC

Plasmid

#176873

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_B4800EC

Plasmid

#176874

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAG2

Plasmid

#176864

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG3

Plasmid

#176866

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_3

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG4

Plasmid

#176867

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos2-del6-22_X

Plasmid

#147961

Purpose

Mammalian Expression of HsNanos2-del6-22

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2-del6-22 (NANOS2 Human)

Expression

Mammalian

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP206-AAV-1.3CaMKII-MCS-pA.

Plasmid

#78319

Purpose

AAV vector backbone containing a 1.3CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Promoter

1.3CaMKIIa

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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