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pVC0*_D_02_1C-5C sfGFP

Plasmid

#250039

Purpose

dropout part, lv0*, 1R-5O, sfGFP

Depositor

Georg Fritz

Publication

Faber et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

dropout part, lv0*, 1R-5O, sfGFP

Use

Synthetic Biology

Available since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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APEX2-LPETG in pET-Duet-1

Plasmid

#254276

Purpose

Expresses APEX2-LPETG in bacterial cells

Depositor

Mia Huang

Publication

Vilen et al ACS Chem Biol. 2023 Apr 21;18(4):701-710

Insert

APEX2

Tags

His tag

Expression

Bacterial

Available since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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pVC0*_D_01_1R-5O sfGFP

Plasmid

#250038

Purpose

dropout part, lv0*, 1C-5C, sfGFP

Depositor

Georg Fritz

Publication

Faber et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

dropout part, lv0*, 1C-5C, sfGFP

Use

Synthetic Biology

Available since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx2_SpRY (ABE8e_L34W_nCas9_SpRY)

Plasmid

#251545

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the L34W mutation in the deaminase domain

Depositor

George Church

Publication

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_L34W-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx2 L34W

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pTG565

Plasmid

#237395

Purpose

Peft3::TIR1 (ChrIV Repair Template with SEC)

Depositor

Ariel Pani

Publication

Gibney et al Genetics. 2026 Jan 20:iyag016. doi: 10.1

Insert

Peft3>TIR1 (ChrIV Repair Template with SEC)

Available since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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eOYW006

Plasmid

#238387

Purpose

TurboRFP constitutive Golden Gate construct

Depositor

Richard Murray

Publication

Larsson et al ACS Synth Biol. 2025 Oct 17;14(10):4122-

Insert

UNS1-P3m-U3d-TurboRFP-t0-UNSX

Expression

Bacterial

Available since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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eOYW008

Plasmid

#238389

Purpose

TurboRFP constitutive Golden Gate construct

Depositor

Richard Murray

Publication

Larsson et al ACS Synth Biol. 2025 Oct 17;14(10):4122-

Insert

UNS1-P4m-U3d-TurboRFP-t0-UNSX

Expression

Bacterial

Available since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-gLuc-DR_pCMV-RedFireFly.gb

Plasmid

#242435

Purpose

Gaussia Luciferse with a Direct Repeat sequence in the 3’UTR recognised by the CasRx under the control of the CMV promoter, and a RedFirefly Luciferase driven by a CMV promoter.

Depositor

Diego Di Bernardo

Publication

De Carluccio et al Nat Commun. 2024 Apr 17;15(1):3311. doi:

Insert

Gaussia Luciferase & Red Firefly Luciferase

Use

CRISPR and Luciferase

Available since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pQE30-His-LZ3B-T7RNAP

Plasmid

#247367

Purpose

Express LZ3B-T7RNAP that dimerize with LZ3A

Depositor

Sebastian Maerkl

Publication

Lee et al bioRxiv 2025.06.18.660355

Insert

LZ3B-T7 RNAP

Tags

His

Expression

Bacterial

Promoter

T5-lacO

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-21(+)-His-zf438-NB23

Plasmid

#247372

Purpose

Express zf438-NB23 that bind to SARS-CoV-19 Spike protein cooperatively with NB15-T7 RNAP.

Depositor

Sebastian Maerkl

Publication

Lee et al bioRxiv 2025.06.18.660355

Insert

His-zf438-NB23

Tags

6xHis

Expression

Bacterial

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PKT2/CLP-Aoah

Plasmid

#239612

Purpose

Expresses mouse Aoah protein in the transposon vector

Depositor

Xin-yuan Guan

Publication

Gong et al Cell. 2025 Jul 24:S0092-8674(25)00793-7.

Insert

Acyloxyacyl Hydrolase (Aoah Mouse)

Use

Transposon

Tags

HA

Expression

Mammalian

Available since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK1058

Plasmid

#209548

Purpose

BTK Type 1 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Publication

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Upstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)

Use

Synthetic Biology

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacD-23003

Plasmid

#109183

Purpose

Plasmid donor for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Publication

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Expression

Insect

Promoter

minimal HSP70 promoter

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacD-23005

Plasmid

#109185

Purpose

Plasmid donor for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Publication

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Expression

Insect

Promoter

minimal HSP70 promoter

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mNeonGreen-G418

Plasmid

#236484

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on G418.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mNeonGreen

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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p371_DG

Plasmid

#234694

Purpose

Golden gate domesticated pET28b with Intein-Chitin binding domain

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

Intein-Chitin binding domain

Expression

Bacterial

Mutation

No

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mCherry-G418

Plasmid

#236490

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on G418.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mCherry

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-Dendra2-G418

Plasmid

#236496

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on G418.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

Dendra2

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mScarlet-G418

Plasmid

#236493

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on G418.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mScarlet

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-GFP-G418

Plasmid

#236478

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on G418.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

GFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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