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5xGMR

Plasmid

#165775

Purpose

5xGMR syntethic eye driver enhancer, requires minimal promoter element

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

5xGMR

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDmActin5c

Plasmid

#165777

Purpose

Drosophila Actin 5C promoter

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Actin5c

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ134-STU-As

Plasmid

#138103

Purpose

Golden Gate entry vector for 4th crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ134-STU-Fn

Plasmid

#138104

Purpose

Golden Gate entry vector for 4th crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ131-STU-As

Plasmid

#138094

Purpose

Golden Gate entry vector for 1st crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ131-STU-Fn

Plasmid

#138095

Purpose

Golden Gate entry vector for 1st crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ132-STU-Fn

Plasmid

#138098

Purpose

Golden Gate entry vector for 2nd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ133-STU-Fn

Plasmid

#138101

Purpose

Golden Gate entry vector for 3rd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pMiniwhiteA1

Plasmid

#165872

Purpose

Basic transgenesis Mini-white physical eye marker cassette in Alpha1, lacks attB site

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pIM16

Plasmid

#132583

Purpose

pZA4-oxySp-mCherry-pLsoxS-mCherry

Depositor

Timothy Lu

Publication

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Inserts

oxySp-mCherry-TermT1
pLsoxS-mCherry-TermT1

Expression

Bacterial

Promoter

oxySp and pLsoxS

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pIM09

Plasmid

#132582

Purpose

pZE1-oxySp-sfGFP-proD-oxyR

Depositor

Timothy Lu

Publication

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Inserts

oxySp-sfGFP-TermT1
proD-oxyR-TermT1

Expression

Bacterial

Promoter

oxySp and proD

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM65905

Plasmid

#153217

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBW002

Plasmid

#131591

Purpose

Vector with aTc inducible M. laminosus Fd1 without BbsI restriction sites in the vector backbone

Depositor

Jonathan Silberg

Publication

Wu et al bioRxiv May 23, 2019

Insert

Mastigocladus laminosus ferredoxin

Use

Synthetic Biology

Available since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBC2102-reci

Plasmid

#117171

Purpose

U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLS

Depositor

David Savage

Publication

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_wtCas9 without NLS

Use

Lentiviral

Promoter

U6 Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pZS2katGp-RBS31-PhiC31-proD-oxyR

Plasmid

#78213

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pNX3s2-HA3

Plasmid

#78396

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA
zeoCV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3a-FL3

Plasmid

#78358

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFLAG
kanMX6

Expression

Yeast

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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EZH2-TALEN_Left_No_FLAG

Plasmid

#79899

Purpose

Untagged EZH2-TALEN_Left to target the N-terminus of human EZH2

Depositor

Yannick Doyon

Publication

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

EZH2-TALEN_Left_No_FLAG

Use

TALEN

Expression

Mammalian

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pBTR(SMCytc)

Plasmid

#72330

Purpose

Expresses spider monkey cytochrome c along with the heme lyase (CYC3) from yeast for heme insertion

Depositor

Bruce Bowler

Insert

spider monkey cytochrome c gene

Expression

Bacterial

Available since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only
pYTK093

Plasmid

#65200

Purpose

Encodes LEU2 5' Homology as a Type 8b part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

LEU2 5' Homology

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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