We narrowed to 17,432 results for: tes

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lentiSAMv2 EGFP-guide2

Plasmid

#118167

Purpose

CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA2 against the EGFP CDS

Depositor

Jorge Ferrer

Publication

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Braf R188L

Plasmid

#118338

Purpose

Expression of human Braf

Depositor

Philip Stork

Publication

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Braf R188L (BRAF Human)

Tags

Flag

Expression

Mammalian

Mutation

R188L

Promoter

CMV

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDONOR-PARK6e5-I368N

Plasmid

#86154

Purpose

Donor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP and dTomato

Depositor

Jens Schwamborn

Publication

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

PINK1 exon 5 homology arms (PINK1 Human)

Expression

Bacterial and Mammalian

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pEB5 (mANGPTL8-V5-His lenti)

Plasmid

#109115

Purpose

V5 and His-tagged mouse ANGPTL8 in a lentiviral vector

Depositor

Brandon Davies

Publication

Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

Insert

ANGPTL8 (Angptl8 Mouse)

Use

Lentiviral

Tags

6x His and V5

Expression

Mammalian

Promoter

CMV

Available since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 kif26b sgRNA1

Plasmid

#102857

Purpose

Expresses sgRNA targeting mouse Kif26b

Depositor

Henry Ho

Publication

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Mouse Kif26b (Kif26b Mouse)

Use

Lentiviral

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 kif26b sgRNA2

Plasmid

#102858

Purpose

Expresses sgRNA targeting mouse Kif26b

Depositor

Henry Ho

Publication

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Mouse Kif26b (Kif26b Mouse)

Use

Lentiviral

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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HA-human-BNIP3 delta Exon2

Plasmid

#100782

Purpose

Mammalian expression of BNIP3 delta Exon2 splice variant

Depositor

Joseph Gordon

Publication

Field et al Cell Death Discov. 2018 Sep 21;4:37. doi

Insert

human-BNIP3 delta Exon2 (BNIP3 Human)

Tags

HA

Expression

Mammalian

Mutation

Exon2 deletion

Promoter

CMV

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_LN-HA-AGO2-5xE

Plasmid

#92011

Purpose

Expresses Lambda-N-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

Lambda-N-HA

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E (5xE)

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-S828A

Plasmid

#91982

Purpose

Expresses FLAG-HA-AGO2 (S828A)

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828A

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAG415-GPDp-EGFP-rap1-K3NR

Plasmid

#84533

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,117,118)R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K3CR

Plasmid

#84534

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(462,463,651)R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K9R

Plasmid

#84535

Purpose

This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K651R

Plasmid

#84536

Purpose

This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K651R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pILGFP95

Plasmid

#83550

Purpose

Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

PDA1 promoter-yEGFP

Expression

Yeast

Promoter

PDA1

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only
pILGFP86

Plasmid

#83543

Purpose

Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL4A promoter-yEGFP

Expression

Yeast

Promoter

RPL4A

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K462-463-651R

Plasmid

#84564

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K462-463-651R

Promoter

none

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K43-117-118R

Plasmid

#84565

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K43-117-118R

Promoter

none

Available since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFPH3

Plasmid

#83553

Purpose

Used to evaluate the expression output of HXT7 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

HXT7 promoter-yEGFP

Expression

Yeast

Promoter

HXT7

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP84

Plasmid

#83542

Purpose

Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

SSA1 promoter-yEGFP

Expression

Yeast

Promoter

SSA1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rNanog-pgk-hph

Plasmid

#74917

Purpose

Mammalian expression of rNanog

Depositor

Jose Silva

Publication

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nanog (Nanog Rat)

Expression

Mammalian

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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