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pGEX Mist-SH2 RK

Plasmid

#46455

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist RK (Clnk Mouse)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Grb2-SH2 RK

Plasmid

#46441

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb2 RK (GRB2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 58-159

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgATXN1L-2

Plasmid

#74971

Purpose

sgATXN1L-2, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ATXN1L (ATXN1L Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-REX-GECO0.9

Plasmid

#61249

Purpose

Expresses REX-GECO0.9 in neurons

Depositor

Robert Campbell

Article

Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

Insert

REX-GECO0.9

Use

AAV

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1: P60R, V61W, R6…

Promoter

hSyn

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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TRBV16

Plasmid

#123451

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV16 (TRBV16 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-RanV19 E1RA2

Plasmid

#118317

Purpose

Expression of human Ran

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Inserts

RanV19 (RAN Human)
EPac1RA2 (RAPGEF3, RAPGEF4 Human)

Tags

GFP

Expression

Mammalian

Mutation

in-frame insertion of RanV19 into GFPEpac1RA2 and…

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Arg-SH2

Plasmid

#46405

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Arg/Abl2 (ABL2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 151-257

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX chimerin-SH2

Plasmid

#46415

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

chimerin (CHN1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 44-147

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Dapp1-SH2

Plasmid

#46422

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Dapp1 (DAPP1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 25-151

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Mist-SH2

Plasmid

#46454

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist (Clnk Mouse)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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p3-HPRT1-S104Rf-PdTK-mCh

Plasmid

#107277

Purpose

HPRT1 c.312C>A (Munich) donor vector with bilateral microhomology

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Inserts

HPRT genomic DNA (HPRT1 Human)
puro-deltaTK; CAG::mCherry

Mutation

c.306G>T, c.312C>A

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PPARgamma2 2KR

Plasmid

#78772

Purpose

To overexpress PPARgamma2 2KR in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPAR_2 (Ppargc1a Mouse)

Expression

Mammalian

Mutation

K184A, K185A

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pCS252

Plasmid

#33207

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

MAP4K4 3'UTR (MAP4K4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-CGCG sites: aaCgCga, aaCgCgaa

Promoter

SV40 early enhancer/promoter

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pX459-TMEM217-gRNA#1

Plasmid

#249417

Purpose

pX459-gRNA#1 targeting the 5′ coding region of human TMEM217

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

TMEM217 (TMEM217 Human)

Use

CRISPR

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pX459-TMEM217-gRNA#2

Plasmid

#249418

Purpose

pX459 plasmid containing gRNA#2 targeting the end of the coding exon of human TMEM217

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

TMEM217 (TMEM217 Human)

Use

CRISPR

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-mouse Tmem217-3xFlag

Plasmid

#249409

Purpose

Expression vector of mouse Tmem217 tagged with 3xFLAG at C-terminus under the CAG promoter.

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

Transmembrane protein 217 (Tmem217 Mouse)

Expression

Mammalian

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-mouse Slc9c1-PA

Plasmid

#249412

Purpose

Expression vector of mouse SLC9C1 tagged with PA at C-terminus under the CAG promoter

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

Slc9c1 (Slc9c1 Mouse)

Tags

PA

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLCV2-AAVS1(hU6-sg1-mU6-sg2)-Blast

Plasmid

#208343

Purpose

pLentiCRISPRv2-Blast backbone with two separate sgRNAs against AAVS1. Serves as a negative control by targeting a safe harbor locus.

Depositor

Peter S Nelson

Article

Frank et al eLife 2025

Insert

AAVS1 (AAVS1 Human)

Use

CRISPR and Lentiviral

Promoter

hU6, mU6

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pCR8-CDK12

Plasmid

#208347

Purpose

Gateway cloning entry vector with CDK12. Must be recombined into a DEST vector for expression.

Depositor

Peter S Nelson

Article

Frank et al eLife 2025

Insert

cyclin dependent kinase 12 (CDK12 Human)

Use

Gateway: entry vector

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab003-pcDNA3.1-FKBP5-3xFLAG

Plasmid

#249006

Purpose

This plasmid expresses the C terminally 3x-FLAG tagged FKBP5 protein sequence for pulldown assays

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

FKBP5 (FKBP5 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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