We narrowed to 18,381 results for: URE

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pCellFree_G03 GTF2H1

Plasmid

#67108

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2H1 (GTF2H1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pmCherry_C1 I3(resist)

Plasmid

#61399

Purpose

Expresses mCherry tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Tags

mCherry

Expression

Mammalian

Mutation

silent mutations of bases C183T T192C T189C

Promoter

CMV

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-GFP-I3(resist)

Plasmid

#61400

Purpose

Expresses GFP-tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Tags

GFP

Expression

Mammalian

Mutation

silent mutations of bases C183T T192C T189C

Promoter

CMV

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-GFP-I3(V41A/W43A)

Plasmid

#61402

Purpose

Expresses GFP-tagged, PP1 binding-deficient Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

inhibitor-3 (PPP1R11 Human)

Tags

GFP

Expression

Mammalian

Mutation

V41A/W43A

Promoter

CMV

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
mKO-Vinculin-23

Plasmid

#57852

Purpose

Localization: Focal Adhesions, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Vinculin (VCL Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV10/3xflag-Clock L113E

Plasmid

#47339

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

L113E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCMV10/3xflag-Clock F122D

Plasmid

#47340

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

F122D

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA/BmalF423R

Plasmid

#47348

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

F423R

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pDM10

Plasmid

#41759

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc Fly, E. coli)

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C45S C338S A378C C451S

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pDM8

Plasmid

#41758

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc Fly, E. coli)

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C45S C338S S364C C451S

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pOU1

Plasmid

#41753

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc Fly, E. coli)

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C338S C451S

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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XE224 human flag-Chibby-pCS3+FLT

Plasmid

#16824

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

flag-Chibby (CBY1 Human)

Use

Xenopus expression

Tags

flag

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro shSlp4-a #2

Plasmid

#40070

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp4-a shRNA-2

Use

Lentiviral

Promoter

U6

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro shSlp4-a #1

Plasmid

#40069

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp4-a shRNA-1

Use

Lentiviral

Promoter

U6

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pET-MRPL15

Plasmid

#31345

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

mitochondrial ribosomal protein L15 mature form (MRPL15 Human)

Tags

His

Expression

Bacterial

Mutation

Has the amino acids in the predicted mature form …

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
23SrRNA.RAV12(wt).2508-2580.GAAAC.G2553U

Plasmid

#26191

Depositor

David McKay

Article

Hardin et al J Mol Biol. 2010 Sep 17;402(2):412-27. d

Insert

23S ribosomal RNA fragment (modified) with G2553U mutation

Expression

Bacterial

Mutation

fragment 2508-2580 of E. coli rRNA, with nucleoti…

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pIS58 TIP120A 3'UTR

Plasmid

#14501

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

TIP120A 3'UTR (CAND1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pJK048

Plasmid

#75230

Purpose

Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

Depositor

T. J. Kappock, JoAnne Stubbe

Article

Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

Inserts

N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase

Expression

Bacterial

Mutation

Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

Promoter

T7 and n/a

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2(R53A)-Venus

Plasmid

#138197

Purpose

FRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(R53A)-Venus (Rapgef4 Synthetic, Mouse)

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains arginine-to-alanine substitution at resi…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]

Plasmid

#138198

Purpose

FRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Synthetic, Mouse)

Tags

Cerulean and cpV[E172]

Expression

Mammalian

Mutation

Contains arginine-to-alanine substitution at resi…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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