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pAAVS1P-iCLHN

Plasmid

#66579

Purpose

AAVS1 safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Nanoluc-HaloTag

Expression

Mammalian

Promoter

CAG

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GSDMD-eGFP-SV40p-BlasR

Plasmid

#228254

Purpose

pLenti-CMV-GSDMD-eGFP-SV40p-BlasR plasmid for ectopic expression of human full-length GSDMD fusion with GFP

Depositor

Judy Lieberman

Article

Fontana et al Cell. 2024 Sep 2:S0092-8674(24)00898-5.

Insert

GSDMD (GSDMD Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL Blast Snail ER

Plasmid

#18798

Depositor

Bob Weinberg

Article

Mani et al Cell. 2008 May 16. 133(4):704-15.

Insert

snail (SNAI1 Human)

Use

Retroviral

Tags

ER hormone binding domain

Expression

Mammalian

Mutation

G525R mutation in the ER. The presence of the G52…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pCAG-mScarlet-Giantin

Plasmid

#196872

Purpose

Expresion of Giantin-fused to mScarlet. Used as Golgi marker

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mScarlet-Giantin (GOLGB1 Synthetic, Human)

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph18-eGFP-CCR5TC

Plasmid

#135529

Purpose

Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph18

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro CXCR4 siRNA-2

Plasmid

#12272

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

CXCR4 siRNA (CXCR4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-YTHDF2

Plasmid

#238975

Purpose

Expresses YTHDF2

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

YTHDF2 (YTHDF2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph15-eGFP-CCR5TC

Plasmid

#135528

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-dCas9-P2A-EGFP

Plasmid

#188898

Purpose

Human lentiviral vector for expression of dCas9 with a C-terminal HA-2xNLS and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

dCas9

Use

Lentiviral

Tags

HA-2xNLS and P2A-GFP

Promoter

SFFV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-InPTapp-Kras

Plasmid

#235999

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to plasma membrane.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

InPTapp-Kras (PLEKHA1 Synthetic, Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-InPGrp-Kras

Plasmid

#236001

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to plasma membrane.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

InPGrp-Kras (Cyth3 Synthetic, Rat)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pPBpuro-NRG1-ScNeo

Plasmid

#209906

Purpose

To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

NRG1-ScNeo (NRG1 Human)

Tags

mNeonGreen and mScarlet

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-enCas12a-A3A-Y130F

Plasmid

#193652

Purpose

Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLb-enCas12a-hA3A-Y130F, EGFP

Expression

Mammalian

Mutation

dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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MSP2443

Plasmid

#72252

Purpose

Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBa-KIF5C 559-tdTomato-FKBP

Plasmid

#64211

Purpose

KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Kif5c (Kif5c Rat)

Tags

FKBP and tdTomato

Expression

Mammalian

Mutation

motor domain aa1-559

Promoter

chicken Beta Actin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-mEos3.2-CCR5TC

Plasmid

#135532

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mEos3.2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

mEos3.2

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE3G-human cGAS

Plasmid

#186853

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS without tags (Adamts1 Human)

Use

Retroviral

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-mNeonGreen-CCR5TC

Plasmid

#135534

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

mNeonGreen

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCELF4-4405

Plasmid

#115466

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shCELF4-4405 (CELF4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-S1-GCaMP6f

Plasmid

#160722

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S1-GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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