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pSPgRNA_Ex3_4

Plasmid

#210628

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro-shTAL1

Plasmid

#210640

Purpose

Knockdown of TAL1 endogenous (3'UTR)

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 3' UTR gRNA (TAL1 Human)

Use

Lentiviral

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57-Cx26G45E-IRES-GCaMP6s

Plasmid

#188237

Purpose

3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 variant p.G45E and GCaMP6s

Depositor

Fabio Mammano

Article

Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

Insert

GJB2 (GJB2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

p.G45E

Promoter

Tight TRE promoter

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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trLATm1PtoLmEos3.2

Plasmid

#203749

Purpose

Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Prolines P8 and P17 in the LAT transmembrane domain mutated to L. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trLATm1 (PtoL) (LAT Human)

Tags

mEos3.2

Expression

Mammalian

Mutation

P8L, P17L

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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MT2A_beta-Villin

Plasmid

#200301

Purpose

Bacterial expression plasmids for production of recombinant fusion protein having beta-domain of metallothionein 2a and chicken Villin headpiece domain HP-35

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

beta-domain of metallothionein 2a in fusion with HP-35 (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Promoter

T7

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMeCP2G pc1121

Plasmid

#167566

Purpose

The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced GFP (isolated from pEGFP-N1 vector; CLONTECH Laboratories, Inc.)

Depositor

Cristina Cardoso

Article

Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

Inserts

Methyl-CpG binding protein 2 (Mecp2 Rat)
GFP

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3a- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192236

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3a

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3a-knockin-homology arm-mAID-mCherry2-Hygro (EIF3A Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3a- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192235

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3a

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3a-knockin-homology arm-mAID-mClover-NeoR (EIF3A Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192243

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3k

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3k-knockin-homology arm-mAID-mClover-NeoR (EIF3K Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192244

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3k

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3k-knockin-homology arm-mAID-mCherry2-Hygro (EIF3K Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192237

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3b

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3b-knockin-homology arm-mAID-mClover-NeoR (EIF3B Human)

Expression

Mammalian

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192238

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3b

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3b-knockin-homology arm-mAID-mCherry2-Hygro (EIF3B Human)

Expression

Mammalian

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pT22iU66CriT(#387)

Plasmid

#184061

Purpose

cyclofen-inducible CRE activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

Insert

CRE-ERT2

Use

Zebrafish transgenesis (blue eyes)

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET-UTX(420-633)-H6

Plasmid

#183773

Purpose

Express pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

Insert

UTX

Tags

6 His tag at the C-terminus

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-CBP(1603-2678)

Plasmid

#183774

Purpose

Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

Insert

nej (nej Fly)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His10-BTLAicd (AA190-289)-TwinStrep

Plasmid

#180805

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289)-TwinStrep (BTLA Human)

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-2-GST-PD-1icd (AA194-288)

Plasmid

#180814

Purpose

Expression of GST fused human PD-1 intracellular domain (AA194-288)

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

PD-1int (AA194-288) (PDCD1 Human)

Expression

Bacterial

Promoter

Tac

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHJ4

Plasmid

#158785

Purpose

Pan-neuronal nuclear-localized GCaMP expression in C. elegans

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Prab-3::nls::GCaMP6m

Tags

NLS

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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LLP245 pEF1a-TALE-DNMT3a-Wt

Plasmid

#100937

Purpose

Empty TALE vector driven by EF1a promoter, no Puro, contains DNMT3a catalytic domain

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

TALE insertion site, DNMT3a catalytic domain (DNMT3A Human, Synthetic)

Use

TALEN

Tags

3xHA and 3xTy1

Expression

Mammalian

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRSM4

Plasmid

#101642

Purpose

Encodes the fluorescent protein KCyG4219 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

KCyG4219

Tags

6XHis

Expression

Bacterial

Mutation

C-terminal residues (221-223) deletion. Ser220Val…

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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