We narrowed to 56,414 results for: ELL

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pDEST-CMV-N-Tandem-mCherry-EGFP

Plasmid

#123216

Purpose

N-terminal Tandem mCherry EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-i53-bpA-EF1BFP

Plasmid

#111145

Purpose

Expression of i53 and a BFP reporter in mammalian cells

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Inserts

i53 (mutL Synthetic)
BFP (BFP Synthetic)

Expression

Mammalian

Mutation

human Ubiquitin, contains 8 point mutations

Promoter

CAG promoter and EF1

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLX317-QKI

Plasmid

#115441

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

QKI (QKI Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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mOPN-pcDNA

Plasmid

#106447

Purpose

Mammalian expression of murine osteopontin

Depositor

Georg Weber

Article

Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

Insert

osteopontin (Spp1 Mouse)

Expression

Mammalian

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)

Plasmid

#47870

Purpose

All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Inserts

NmCas9
U6pr-tracrRNA
OCT4 crRNA

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Available Since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pIND-hygro-HA-H3.3

Plasmid

#47981

Purpose

Ponasterone A-inducible expression of human HA tagged H3F3A

Depositor

Bing Zhu

Article

Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:

Insert

H3F3A (H3-3A Human)

Tags

HA

Expression

Mammalian

Mutation

288 bp T to C synonymous mutation

Promoter

Minimal Heat Shock Promoter

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
CLPIT Cry2PHR-mCh-LRP6c

Plasmid

#42961

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-LRP6c

Use

Retroviral

Expression

Mammalian

Mutation

LPR6 cytoplasmic domain only

Promoter

Tet-Off promoter

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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p6618 MSCV-IP N-HAonly WHSC2

Plasmid

#35014

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

WHSC2 (NELFA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 9, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CTLA4-mgSrtA

Plasmid

#125794

Purpose

for co-expression of CTLA4 and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD80/86 positive cells.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

CTLA4-mgSrtA

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-ZHER-mgSrtA

Plasmid

#125793

Purpose

for co-expression of ZHER (a HER2 binding affibody) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of HER2 positive cells.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

ZHER-mgSrtA

Expression

Mammalian

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-PE-Plus

Plasmid

#214079

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

PE-Plus

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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PB-TRE-OKSM

Plasmid

#204498

Purpose

Expresses Oct4, Klf4, Sox2 and c-Myc by tet-on promoter in mammalian cells

Depositor

Wataru Fujii

Article

Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

Inserts

OCT4 (POU5F1 Human)
KLF4 (KLF4 Human)
SOX2 (SOX2 Human)

Expression

Mammalian

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-dCas9-KRAB-GFP backbone

Plasmid

#194281

Purpose

Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Promoter

EF1a

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag

Plasmid

#132407

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dLwaCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R474A; H479A; R1046A; R1051A

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-IV-GFPL10

Plasmid

#98704

Purpose

Cre-dependently express GFPL10 fusion using Introvert Switch

Depositor

Jeffrey Friedman, Alexander Nectow

Article

Nectow et al Cell. 2017 Jul 27;170(3):429-442.e11. do

Insert

GFPL10

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

L10: 2609 C->T to remove EcoRI site

Promoter

EF1a

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 C506S

Plasmid

#78584

Purpose

Expression flag-USP19 C506S in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 C506S (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

mutate 506 cysteine residue to serine

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pTD-C_sfYFPTwinStrep

Plasmid

#45943

Purpose

expresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion protein

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Insert

synthetic sfYFP (codon usage adapted to P.putida KT2440)

Tags

Twin-Strep-tag

Expression

Bacterial

Promoter

lacIq/Ptrc

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-GFP-PTEN-WT

Plasmid

#30391

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

GFP-PTEN-WT (PTEN Human)

Expression

Mammalian

Mutation

GFP-PTEN-WT

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3

Plasmid

#73208

Purpose

Expresses dL5(E52D)-mCer3 fusion protein in mitochondria of mammalian cells. (MBIC5, dL5**, FAP)

Depositor

Marcel Bruchez

Article

Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

Insert

COXIV-COX8-dL5-2XG4S-mCer3 (COX8A Synthetic, Human)

Tags

The FAP and mCerulean3 are fused with 2 copies of…

Expression

Mammalian

Mutation

The dL5** FAP is E50D, L89S, also known as E52D/L…

Promoter

CMV

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pAH123

Plasmid

#66077

Purpose

The pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 version

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

phi80 integrase

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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