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Plasmid#174804PurposeBacterial expression of an inactive PARP1 mutant lacking the ART subdomainDepositorInsertPARP1deltaART (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 785-1014PromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn1
Plasmid#173933PurposeBacterial expression of PARP1 lacking Zn1 domainDepositorInsertPARP1delZn1 (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 1-96; V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn2
Plasmid#173935PurposeBacterial expression of PARP1 lacking Zn2 domainDepositorInsertPARP1delZn2 (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 97-213 (V762A)PromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-ART
Plasmid#174810PurposeBacterial expression of isolated PARP1 ART subdomainDepositorInsertPARP1-ART (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-BRCT
Plasmid#174807PurposeBacterial expression of isolated PARP1 BRCT domainDepositorInsertPARP1-BRCT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-WGR
Plasmid#174808PurposeBacterial expression of isolated PARP1 WGR domainDepositorInsertPARP1-WGR (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-HD
Plasmid#174809PurposeBacterial expression of isolated PARP1 HD subdomainDepositorInsertPARP1-HD (PARP1 Human)
UseTags6xHisExpressionBacterialMutationV762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hPPP3R1
Plasmid#179136PurposeExpression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Y986S
Plasmid#174802PurposeBacterial expression of a PARP1 mutant that produces mainly short PAR oligomersDepositorInsertPARP1-Y986S (PARP1 Human)
UseTags6xHisExpressionBacterialMutationY986H, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-G972R
Plasmid#174800PurposeBacterial expression of a PARP1 mutant that produces less PAR oligomers overallDepositorInsertPARP1-G972R (PARP1 Human)
UseTags6xHisExpressionBacterialMutationG972R, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only