-
Plasmid#144188PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTADA2B (TADA2B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRB_Hormone-recep
Plasmid#109909PurposeProtein expression and purification of ESRRB_Hormone-recepDepositorInsertESRRB_Hormone-recep (ESRRB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-NLS-actin-R62D
Plasmid#118381PurposeGateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.DepositorInsertactin beta (ACTB Human)
UseDonor-plasmidTagsExpressionMutationNLS and R62DPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-NLS-actin
Plasmid#118380PurposeGateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.DepositorInsertactin beta (ACTB Human)
UseDonor-plasmidTagsExpressionMutationNLSPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2AL200R150G-EGFP-LC3B-RFP-LC3BΔG
Plasmid#168998PurposeExpresses GFP-LC3-RFP-LC3ΔG in zebrafish to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseUnspecifiedTagsEGFP and mRFP1ExpressionMutationPromoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
p221a-GUS
Plasmid#71263PurposeEntry clone containing the GUS enzyme. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertBeta-glucuronidase
UseGatewayTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
GLuc-PM
Plasmid#164783PurposeGaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorInsertGaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Gaussia princeps, Human)
UseTagsGLucExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
NanoLuc-PM
Plasmid#164784PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Oplophorus gracilirostris)
UseTagsNanoLucExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-FLAG-TAOK2
Plasmid#197862PurposeExpression of FLAG-tagged TAOK2 / PSK1 alpha in mammalian cellsDepositorInsertTAOK2 (TAOK2 Human)
UseTagsGFPExpressionMammalianMutationWild TypePromoterSV40Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
NanoLuc-PM-Venus
Plasmid#164786PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent proteinDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Oplophorus gracilirostris)
UseTagsNanoLuc and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shSTING-Mmu
Plasmid#127697PurposeDoxycyclin inducible shRNA knockdown of mouse STING geneDepositorInsertshSTING Mmu (Sting1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
GLuc-PM-Venus
Plasmid#164785PurposeGaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent proteinDepositorInsertGaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Gaussia princeps, Human)
UseTagsGLuc and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-β2AR-μDpTT
Plasmid#182326PurposeBeta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertbeta2AR-μDronpa-TagRFP-T (ADRB2 Human)
UseTagsFull-length human beta2 adrenergic receptorExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330-mitf
Plasmid#69801PurposeUsed to make mitf knockout porcine cell lineDepositorInsertsmitf-sgRNA
SpCas9
UseTagsExpressionMammalianMutationPromoterChicken Beta-Actin and U6Available sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
WTTEM1_pSTC0-zeo
Plasmid#202351PurposeControl WT TEM-1 (wild type TEM-1 beta lactamase) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsertTEM-1
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-STING(pLxIS)
Plasmid#221333PurposeOverexpression of STING CTT in bacteriaDepositorInsertSTING CTT (Sting1 Mouse)
UseTags6xHis-4xFKBPExpressionBacterialMutationPromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-STING(pLxIS)
Plasmid#221240PurposeExpression of STING CTT in mammalian cells by retroviral transductionDepositorInsertSTING CTT (Sting1 Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBF_HsPepT1
Plasmid#73943PurposeXenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tagDepositorInsertPepT1 (SLC15A1 Human)
UseMake rna for xenopus oocyte injectionTagsFlagExpressionMutationPromoterbeta globinAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBF_HsPepT2
Plasmid#73944PurposeXenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tagDepositorInsertPepT2 (SLC15A2 Human)
UseMake rna for xenopus oocyte injectionTagsFlagExpressionMutationPromoterbeta globinAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330 Ctnnb1.2
Plasmid#59912PurposepX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoterDepositorInsertCtnnb1 (Ctnnb1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only