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p2T-cmv-SpCas9-TadCBEd-V106W-BlastR

Plasmid

#193849

Purpose

Express TadCBEd-V106W in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd-V106W

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 proHB-EGF NC

Plasmid

#11605

Depositor

Rosalyn Adam

Article

Kim et al Cancer Res. 2005 Sep 15. 65(18):8242-9.

Insert

HB-EGF (HBEGF Human)

Expression

Mammalian

Mutation

Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-ABE7.8

Plasmid

#102917

Purpose

expresses ABE7.8 in mammalian cells

Depositor

David Liu

Article

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Insert

ABE7.8

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-CP110

Plasmid

#136802

Purpose

Mammalian expression of the centrosomal protein Cep110 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-CP110 (CCP110 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEa-V106W

Plasmid

#193838

Purpose

Express TadCBEa-V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDa V106W-SpCas9 D10A

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAcBac1-GFP(39TAG)

Plasmid

#217360

Purpose

Expression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmid

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

Enhanced green fluorescent protein

Use

Baculoviral

Expression

Insect and Mammalian

Mutation

Y39TAG

Promoter

CMV

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPyPGK-Myc-LaG17-SynNotch-tTA-IRES-Ble

Plasmid

#183607

Purpose

Mammalian expression vector: mouse Pgk1 promoter driving expression of a SynNotch receptor comprising anti-GFP nanobody (LaG17), mouse Notch1 core, tTA. Confers bleomycin/zeocin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

LaG17 GFP nanobody SynNotch tTA

Tags

Myc and human CD8a signal sequence

Expression

Mammalian

Promoter

Mouse Pgk1

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadCBEa-BlastR

Plasmid

#193847

Purpose

Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEa

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Cep131

Plasmid

#136875

Purpose

Mammalian expression of the centrosomal protein Cep131 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep131 (CEP131 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Intron-CterFlag-MePCE

Plasmid

#113549

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

Flag

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Cep164

Plasmid

#136873

Purpose

Mammalian expression of the centrosomal protein Cep164 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep164 (CEP164 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-ITR-EcYtR(NGS-6)-mCherry

Plasmid

#217363

Purpose

Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

E. coli tyrosine tRNA mutant, "NGS-6"

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR_Mm_CEP55(WT)

Plasmid

#136333

Purpose

Mouse CEP55 cDNA in Gateway pENTR

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Nat Commun. 2018 Aug 1;9(1):3012. doi: 1

Insert

CEP55 (Cep55 Mouse)

Use

Gateway entry vector

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Centrobin

Plasmid

#136829

Purpose

Mammalian expression of the centrosomal protein Centrombin N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrobin (CNTROB Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shJUND puro

Plasmid

#58698

Purpose

Lentiviral shRNA vector for knockdown of human JUND

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

shJUND (JUND Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-TUBG2

Plasmid

#171966

Purpose

Expression in cell lines of the TUBG2 gene

Depositor

Maria Alvarado-Kristensson

Article

Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

Insert

TUBG2 (TUBG2 Human)

Expression

Mammalian

Mutation

Removal of the internal TUBG2-EcoRI site by chang…

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

Plasmid

#176839

Purpose

To recruit SAM complex to human CYP3A4 proximal enhancer.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer sgRNA for activation

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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Erk2

Plasmid

#40777

Purpose

Expression of rat Erk2 in mammalian cells

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

Insert

Erk2 (Mapk1 Rattus norvegicus)

Tags

eGFP

Expression

Mammalian

Mutation

Starting Met removed

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pCDNAIIIB rac1 QL

Plasmid

#61633

Purpose

mammalian expression of rac1 constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

Q61L, constitutively active

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-Cep57

Plasmid

#136817

Purpose

Mammalian expression of the centrosomal protein Cep57 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep57 (CEP57 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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