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Plasmid#216472Purposeto express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start siteDepositorInsertsgRNA targeting human AGER locus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2704-AGER-gRNA3
Plasmid#216473Purposeto express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start siteDepositorInsertsgRNA targeting human AGER locus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2705-AGER-eGFP
Plasmid#216474Purposedonor vector for targeting an eGFP reporter to the human AGER locus at the endogenous ATG start siteDepositorInserteGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP
Plasmid#216477Purposelentiviral dual expression of dsRed and tagBFP with loxP site for Cre excisionDepositorInsertsdsRed
tagBFP
UseCre/Lox and LentiviralTagsExpressionMutationPromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINOH11-A10
Plasmid#184278PurposeExpresses nanobody H11-A10 in E. coliDepositorInsertNanobody H11-A10
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_H11-B5
Plasmid#184279PurposeExpresses nanobody H11-B5 in E. coliDepositorInsertNanobody H11-B5
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_11-H6
Plasmid#184280PurposeExpresses nanobody H11-H6 in E. coliDepositorInsertNanobody H11-H6
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINH11-H4 Q98R_H100E
Plasmid#184281PurposeExpresses nanobody H11-H4 Q98R_H100Ein E. coliDepositorInsertNanobody H11-H4 Q98R_H100E
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS1-TEV-HAT
Plasmid#202557PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cellDepositorInsertSGMS1 (SGMS1 Human)
UseTagsHistidine-Affinity-tag (HAT) and TEV cleavable si…ExpressionMammalianMutationdeleted stop codon (TAA)PromoterCAG and chicken β-actin promoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS2-TEV-Twin-Strep
Plasmid#202529PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cellDepositorInsertSGMS2 (SGMS2 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TGA)PromoterCAG and chicken β-actin promoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-EF-5Flnc
Plasmid#174870PurposeCRISPR vector for generating Flnc STREAMING-tag KI cellDepositorInsertsgRNA for mouse Flnc (Flnc Synthetic)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD437
Plasmid#168463PurposeFor the insertion pf NLS-mEos3.2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mEos3.2-H-NSdbd-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD440
Plasmid#168466PurposeFor the insertion pf NLS-mCherry-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mCherry-H-NSdbd-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD441
Plasmid#168467PurposeFor the insertion pf NLS-mEGFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mEGFP-H-NSdbd-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD442
Plasmid#168468PurposeFor the insertion of NLS-eYFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-eYFP-H-NSdbd-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD443
Plasmid#168469PurposeFor the insertion pf NLS-mScarlet-I-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mScarlet-I-H-NSdbd-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD444
Plasmid#168470PurposeFor the insertion of NLS-mNeonGreen-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mNeonGreen-H-NSdbd-NLS
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
human TLNRD1-4H pET151
Plasmid#159386PurposeExpresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cellsDepositorInsertTalin Rod Domain Containing Protein 1 (TLNRD1 Human)
UseTagshis-tag, TEV cleavage siteExpressionBacterialMutationTLNRD1 4-helix domain (residues 143-273)PromoterT7Available sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSECC-sgSIK1-B
Plasmid#138666PurposeExpresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinaseDepositorInsertsgSIK1 mouse (Sik1 Mouse)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSECC-sgSIK3-A
Plasmid#138672PurposeExpresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinaseDepositorInsertsgSIK3 mouse (Sik3 Mouse)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only