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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,296 results for: cfp

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Showing: 1101 - 1120 of 1296 results

  2. pcDNA3-InPAkt

    Plasmid
    #181914
    Purpose
    Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.
    Depositor
    Jin Zhang
    Publication
    Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    InPAkt
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181914-plasmid-map-sequence-id-363357

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  3. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

    Plasmid
    #114092
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AsCas12a (S542R/K548V/N552R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    S542R, K548V and N552R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114092-plasmid-map-sequence-id-230539

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  4. pCMV CytoBAS

    Plasmid
    #62860
    Purpose
    Fluorescent bile acid sensor (BAS) that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET
    Depositor
    Stan van de Graaf
    Publication
    van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CytoBAS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62860-plasmid-map-sequence-id-103768

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  6. pCMV NucleoBAS

    Plasmid
    #62861
    Purpose
    Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET
    Depositor
    Stan van de Graaf
    Publication
    van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NucleoBAS
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62861-plasmid-map-sequence-id-103771

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  7. mCer-Vamp2(qv/fw)

    Plasmid
    #53238
    Purpose
    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.
    Depositor
    Robert Zucker
    Publication
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VAMP2 (Vamp2 Rat)
    Tags
    4aa-linker and mCerulean
    Expression
    Mammalian
    Mutation
    Mutated Q76 to V; F77 to W.
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)

    Plasmid
    #114079
    Purpose
    CAG promoter expression plasmid for human codon optimized AsBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114079-plasmid-map-sequence-id-231032

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  12. PB-NES-ZapCV2

    Plasmid
    #222950
    Purpose
    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Publication
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-ZapCV2
    Tags
    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    222950-plasmid-map-sequence-id-450583

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  13. 4MT-D3-CPV

    Plasmid
    #58184
    Purpose
    Localization: Mitochondria Ca Biosensor, Excitation: 433 / 515 , Emission: 475 / 528
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    ECFP and Venus
    Expression
    Mammalian
    Available since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    58184-plasmid-map-sequence-id-513396

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  15. Beta-2-adrenergic receptor-mCerulean

    Plasmid
    #66957
    Purpose
    Beta-2-adrenergic receptor tagged with mCerulean on carboxyl terminus
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Beta-2-adrenergic receptor-mCerulean (ADRB2 Human, Aequorea victoria)
    Tags
    mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    66957-plasmid-map-sequence-id-115329

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  17. pcDNA3-RIα-ICUE4

    Plasmid
    #181848
    Purpose
    ICUE4 cAMP sensor tethered to the PKA RIα subunit.
    Depositor
    Jin Zhang
    Publication
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RIα-ICUE4 (RAPGEF3, PRKAR1A Human)
    Tags
    ECFP, PKA RIα, and cpVenus(L194)
    Expression
    Mammalian
    Mutation
    Glutamine 122 in Epac1 domain mutated to glutamat…
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181848-plasmid-map-sequence-id-364152

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Showing: 1101 - 1120 of 1296 results