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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,720 results for: gast

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Showing: 1101 - 1120 of 7720 results

  2. pCaSpeR3/ncd-gfp #10

    Plasmid
    #66082
    Purpose
    stable germline transformation of Drosophila to produce Ncd-GFP expression
    Depositor
    Sharyn Endow
    Publication
    Endow et al J Cell Biol. 1997 Jun 16;137(6):1321-36.
    Insert
    Ncd kinesin-14 motor protein (ncd Fly)
    Tags
    GFP
    Expression
    Insect
    Mutation
    D699L
    Promoter
    ncd promoter (3.1kb upstream region of ncd start …
    Available since
    June 19, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. K560GFP_17NL

    Plasmid
    #129767
    Purpose
    Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    DAL insert before A345
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129767-plasmid-map-sequence-id-263589

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  9. K560_AviN_coBirA

    Plasmid
    #129766
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129766-plasmid-map-sequence-id-263552

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  16. pAc5.1B-lambdaN-HA-DmARC1_N

    Plasmid
    #146978
    Purpose
    Insect Expression of DmARC1
    Depositor
    Elisa Izaurralde
    Insert
    DmARC1 (Arc1 Fly)
    Expression
    Insect
    Mutation
    5 silent mutations and one non silent mutation G3…
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146978-plasmid-map-sequence-id-389936

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  17. pUASTLOTattB_mCherry-CD8-vhhGFP

    Plasmid
    #163930
    Purpose
    Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer
    Depositor
    Markus Affolter
    Publication
    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife
    Insert
    Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Insect
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    163930-plasmid-map-sequence-id-323460

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  18. pcDNA3.1(-)-V5.mER.hOr47a

    Plasmid
    #126473
    Purpose
    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells
    Depositor
    Bill Hansson
    Publication
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    Or47a (Or47a Fly)
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER) and V5 tag
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126473-plasmid-map-sequence-id-246528

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  19. CvcE in pGEX4t

    Plasmid
    #112977
    Purpose
    For protein expression and purification of Drosophila CvcE
    Depositor
    Terry Orr-Weaver
    Insert
    cycE (CycE Fly)
    Expression
    Bacterial
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    112977-plasmid-map-sequence-id-217475

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Showing: 1101 - 1120 of 7720 results