We narrowed to 25,057 results for: Nes

1. pGEMT-PT2A-GFP-Tdtomato-iRFP670

   Plasmid

   #111812

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   GFP
   Tdtomato
   iRFP670
   

   Use

   Cloning intermediate

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMJ825

   Plasmid

   #39315

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine (D10A)

   Promoter

   T7

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRGEB32-BAR

   Plasmid

   #126072

   Purpose

   For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.

   Depositor

   Charles Hunter

   Article

   Hunter et al Plants (Basel). 2021 Apr 8;10(4). pii: p

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…

   Available Since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTrichoGate-5

   Plasmid

   #160705

   Purpose

   Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

   Depositor

   Artemio Mendoza

   Article

   Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

   Insert

   Trichoderma optimised Synthetic eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-Gal80ts

   Plasmid

   #51619

   Purpose

   vertebrate expression of full-length temperature-sensitive Gal80 with stop

   Depositor

   Joshua Bonkowsky

   Article

   Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

   Insert

   Gal80ts (GAL80 Budding Yeast)
   

   Use

   Vertebrate expression

   Tags

   Expression

   Mutation

   temperature sensitive (G203V, G323R)*

   Promoter

   Available Since

   March 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Eef1a-1955/-1>nls::Cas9::nls

   Plasmid

   #59987

   Purpose

   Eef1a (EF1alpha) promoter driving nls::Cas9::nls

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   nls::Cas9::nls
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Reverted mutations from dCas9 to wiltdtype

   Promoter

   Ciinte.Eef1a (EF1alpha) -1955/-1

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMA-SpCas9-g3

   Plasmid

   #80786

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFJ2369 - intron GG1 - 250bp

   Plasmid

   #159877

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   intron GG1 - 250bp
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGreenPuro-shRNA-Stx3S-C4

   Plasmid

   #99742

   Purpose

   3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

   Depositor

   Thomas Weimbs

   Article

   Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

   Insert

   shRNA against Stx3S
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTrichoGate-2

    Plasmid

    #160702

    Purpose

    gpdh promoter from Trichoderma virens bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    gpdh
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Incorporation of BsaI restriction sites

    Promoter

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRN119

    Plasmid

    #176884

    Purpose

    Encodes STM and WUS2 for ectopic meristem induction in plants

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    CmYLCV:Luc+:HSP, Nos::WUS2:pinII, and 35:STM:35S
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNTI661 pRPR1(TetO)-sgRNA

    Plasmid

    #139475

    Purpose

    Vector for tet-regulated sgRNA expression in yeast

    Depositor

    Nicholas Ingolia

    Article

    McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

    Insert

    single guide RNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pRPR1(TetO)

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Sox1/2/3-2373/+3>nls::Cas9::nls

    Plasmid

    #59989

    Purpose

    Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Sox1/2/3 (SoxB1) -2373/+3

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. AW01_pU.CAG.eSpCas9(1.1).rBGpA

    Plasmid

    #199255

    Purpose

    Expression construct encoding the high specificity eSpCas9(1.1) nuclease

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

    Insert

    High specificity eSpCas9(1.1) nuclease
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    K848A K1003A R1060A

    Promoter

    CAG promoter

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122090

    Purpose

    U6 driven SaCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. YFP-NavII-III

    Plasmid

    #26056

    Depositor

    Matthew Grubb

    Article

    Grubb et al Nature. 2010 Jun 24. 465(7301):1070-4.

    Insert

    Nav1.2 (Scn2a Rat)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    The insert codes only for the intracellular loop …

    Promoter

    Available Since

    Oct. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBlu/gRNA

    Plasmid

    #59188

    Purpose

    Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

    Depositor

    Robert Stupar

    Article

    Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

    Insert

    Guide RNA Cassette
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    U6 (Arabidopis)

    Available Since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-Flag YAP S381A

    Plasmid

    #27377

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    S381A

    Promoter

    Available Since

    March 4, 2011

    Availability

    Academic Institutions and Nonprofits only

19. Nanog5P reporter

    Plasmid

    #16337

    Depositor

    Austin Cooney

    Article

    Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

    Insert

    Nanog promoter (Nanog Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Nanog promoter

    Available Since

    Dec. 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-ZnT1-WT

    Plasmid

    #82340

    Purpose

    Expresses FLAG-tagged ZnT1-WT in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

    Insert

    ZnT1-WT (SLC30A1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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