We narrowed to 19,230 results for: REV;

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pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2

Plasmid

#178575

Purpose

Lentiviral vector for GFAP::Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CRY2PHR-P2A-CIBN-mCherry-VAMP2

Use

Lentiviral

Promoter

GFAP

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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KIF20A D5.2 gRNA

Plasmid

#90718

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIF20A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KIF20A (Guide Designation D5.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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ALIX(1-702)-mNeonGreen

Plasmid

#191193

Purpose

Truncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (1-702) (PDCD6IP Human, Synthetic)

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Mutation

Deletion of ALIX C-terminal PRD.

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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FEN1 C10.3 gRNA

Plasmid

#90689

Purpose

3rd generation lentiviral gRNA plasmid targeting human FEN1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FEN1 (Guide Designation C10.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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MASTL B4.4 gRNA

Plasmid

#90755

Purpose

3rd generation lentiviral gRNA plasmid targeting human MASTL

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MASTL (Guide Designation B4.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-TAOK3

Plasmid

#172993

Purpose

constitutive expression of FLAG-tagged TAOK3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-TAOK3 (TAOK3 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 AMSH 1-178

Plasmid

#160050

Purpose

Expresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

AMSH residues 1-178 (STAMBP Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-178

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSaPIPLC His

Plasmid

#173810

Purpose

Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells

Depositor

Anne Gershenson, Mary F Roberts

Article

Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87

Insert

Staphylococcus aureus plc

Tags

6XHis

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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NDUFV3_pcDNA6.2/EmGFP-Bsd

Plasmid

#176946

Purpose

Mammalian expression vector encoding NDUFV3 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

NDUFV3 (NDUFV3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB4 (kinase dead)

Plasmid

#29533

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed lysine 751 to methionine

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
BRCA1 A9.2 gRNA

Plasmid

#90548

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA1 (Guide Designation A9.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Dppa2

Plasmid

#40799

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Dppa2 (Dppa2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
mCerulean3-Caveolin-C-10

Plasmid

#55404

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Caveolin (CAV1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pX330 _CRISPIE donor B10

Plasmid

#172851

Purpose

DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBBK84 pHomL-RPL18Bp-PDR16-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179068

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Scarlet(rfp), a marker for the lipid droplet

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PDR16-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK79 pHomL-RPL18Bp-Scarlet(rfp)-Link-YPT7-SSA1t-HomR (AmpR)

Plasmid

#179063

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-YPT7, a marker for the vacuole, late endosome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-YPT7

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LINXa3

Plasmid

#73614

Purpose

Light-induced nuclear export switch for microscopy in mammalian cells.

Depositor

Brian Kuhlman

Article

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

LINXa3

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

Chicken B-Actin

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-myc-BioID-hp100

Plasmid

#174738

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Article

Li et al Cell Res. 2019 Jun;29(6):474-485. doi: 1

Insert

P100 (NFKB2 Human)

Use

Retroviral

Mutation

WT

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(800-868)

Plasmid

#141345

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868).

Depositor

Lalit Deshmukh

Article

Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

Insert

GB1His6-TEV-ALIX(800-868)

Tags

GB1-His6-TEV

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUBC-EBFP2

Plasmid

#212314

Purpose

Lentiviral backbone with EBFP2 reporter

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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