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pSCAR_sgRNA_puro-mKate-lox5171

Plasmid

#162077

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

Depositor

Robert Manguso

Publication

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pPAiRFP2-N1

Plasmid

#44273

Depositor

Vladislav Verkhusha

Publication

Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

Insert

PAiRFP2

Expression

Mammalian

Mutation

K69R/R83K/G120D/A123T/M163L/Q168E/R220P/S243N/V24…

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/DEST/hCx43-E2HA-stop

Plasmid

#40909

Depositor

Robin Shaw

Publication

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 internal HA tag (GJA1 Human)

Expression

Mammalian

Mutation

HA tag inserted in second extracellular loop

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.Flag.HERC2-HECT-C4762A.6xHis

Plasmid

#39228

Depositor

Aziz Sancar

Publication

Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

Insert

HERC2 HECT Domain (HERC2 Human)

Tags

6xHis and Flag

Expression

Mammalian

Mutation

C4762A

Promoter

T7

Available since

July 31, 2012

Availability

Academic Institutions and Nonprofits only
pQCXIN- myc ULK1 wt

Plasmid

#27626

Depositor

Reuben Shaw

Publication

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 (Myc Mouse)

Use

Retroviral

Tags

myc

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pWZL Neo Myr Flag ADRBK2

Plasmid

#20419

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

ADRBK2 (GRK3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CERK

Plasmid

#20454

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CERK (CERK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGSTag Flag Jnk2a2

Plasmid

#15744

Depositor

Roger Davis

Publication

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a2 (MAPK9 Human)

Expression

Bacterial

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
REEP5-mEmerald

Plasmid

#186964

Purpose

Express REEP5/DP1 with C-terminus mEmerald

Depositor

Ke Xu

Publication

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Reep5 (Reep5 Mouse)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV Promoter

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti CMV TRE3G BCL2L11 Neo

Plasmid

#140747

Purpose

Inducibly expresses BIM in mammalian cells

Depositor

Scott Dixon

Publication

Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

Insert

BCL2L11 (BCL2L11 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV TRE3G

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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Dnase1L3 R206C pIRES-DsRed

Plasmid

#98847

Purpose

Expresses Human Dnase1L3 with activity-reducing HUVS mutation R206C in mammalian cells along with DsRed

Depositor

Peter Keyel

Publication

Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

Insert

Dnase1L3 (DNASE1L3 Human)

Tags

IRES DsRed

Expression

Mammalian

Mutation

R206C which greatly reduces nuclease activity

Promoter

CMV

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pUDE710

Plasmid

#103020

Purpose

expression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)

Depositor

Jean-Marc Daran

Publication

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crADE2-3.crHIS4-4.S (ADE2, HIS4 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-YOD1-C160S

Plasmid

#85666

Purpose

Expression of human YOD1 C160S mutant with N-terminal GFP tag

Depositor

Hemmo Meyer

Publication

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

YOD1 (YOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

C160S

Promoter

CMV

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLXSN-EGFR

Plasmid

#65226

Purpose

Expression of EGFR in mammalian cells

Depositor

Axel Ullrich

Publication

Obermeier et al EMBO J. 1996 Jan 2;15(1):73-82.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only
NG0886 pCI-TPI-WT-4H

Plasmid

#65801

Purpose

NMD reporter mRNA

Depositor

Niels Gehring

Publication

Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

Insert

TPI (TPI1 Human)

Tags

4H (4 copies of short beta-globin 3'UTR for …

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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tdp43-EGFP construct8

Plasmid

#28201

Depositor

Zuoshang Xu

Publication

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa275-313 deleted

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
tdp43-EGFP construct9

Plasmid

#28202

Depositor

Zuoshang Xu

Publication

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa1-193 only

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
tdp43-EGFP construct11

Plasmid

#28204

Depositor

Zuoshang Xu

Publication

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa193-274 only

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-499

Plasmid

#26320

Depositor

David Bartel

Publication

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-499 (MIR499A Human)

Expression

Mammalian

Available since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
mP58.dJ1-pCDNA3

Plasmid

#21884

Depositor

David Ron

Publication

Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

Insert

P58 (Dnajc3 Mouse)

Expression

Mammalian

Mutation

DnaJ domain deleted from C-term

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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