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FXN_Halo_N_allele

Plasmid

#178141

Purpose

Donor vector for endogenous tagging of human FXN at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (FXN Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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GRN_Halo_C_allele

Plasmid

#178125

Purpose

Donor vector for endogenous tagging of human GRN at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (GRN Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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COQ2_Halo_N_allele

Plasmid

#178154

Purpose

Donor vector for endogenous tagging of human COQ2 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (COQ2 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHR: pPGK PYL1-sfGFP-HP1AI165E

Plasmid

#174114

Purpose

Expression of PYL1-sfGFP-HP1AI165E

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

PYL1-sfGFP-HP1AI165E

Use

Lentiviral

Promoter

PGK

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHR: pPGK PYL1-sfGFP-HP1Adel72

Plasmid

#174113

Purpose

Expression of PYL1-sfGFP-HP1Adel72

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

PYL1-sfGFP-HP1Adel72

Use

Lentiviral

Promoter

PGK

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pG-04

Plasmid

#166935

Purpose

Target DC (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

pT181 dual control activator (DC) - Flanked by BsaI restriction sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pG-03

Plasmid

#166934

Purpose

Target AD1.A6 part (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Star sense AD1.S6 - Block G- Flanked by BsaI sites

Use

Synthetic Biology

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pC-04

Plasmid

#166928

Purpose

STAR-DC part (C)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Dual control activator -Block C- Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBoL1P3GA

Plasmid

#112909

Purpose

Express a cloned sgRNA from AtU626 promoter

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2019;1917:155-170. doi

Insert

AtU626-LACZ-sgRNA

Promoter

At U626

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-eCas9

Plasmid

#154925

Purpose

aTc inducible eCas9

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

eCas9

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

K848A, K1003A, R1060A

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-OptiCas9

Plasmid

#154927

Purpose

aTc inducible OptiCas9

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

OptiCas9

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

R661A, K1003H

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-HypaCas9

Plasmid

#154929

Purpose

aTc inducible HypaCas9

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

HypaCas9

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

N692A/M694A/Q695A/H698A

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEPMY1CB0001

Plasmid

#162317

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter.

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0030

Plasmid

#162312

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, stop codon

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0258

Plasmid

#162291

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0025

Plasmid

#162283

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr157

Plasmid

#136753

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr157

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr157

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr272

Plasmid

#136755

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr272

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr272

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr273

Plasmid

#136756

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr273

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr273

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr457

Plasmid

#136757

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr457

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr457

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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