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MP-S3

Plasmid

#69692

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 d80-AAG(Y127I-H136L)

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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EB2316

Plasmid

#87753

Purpose

pkaiBC::EYFP cloned between XbaI and XhoI of EB2065

Depositor

Erin O'Shea

Article

Zheng et al Cell Rep. 2017 Apr 18;19(3):497-504. doi

Insert

pkaiBC::EYFP

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pKM343

Plasmid

#71487

Purpose

Contains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Insert

loxP-hyg-cat-loxP

Use

Source of loxp-hyg-cat- loxp for pcr amplificatio…

Expression

Bacterial

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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MP-K13

Plasmid

#69657

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA polB

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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MP-P7

Plasmid

#69666

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR polB

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pNR-DEST

Plasmid

#90101

Purpose

Gateway destination vector featuring nitrate reductase (NR) promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.

Depositor

Philip Weyman

Article

Bielinski et al PeerJ Preprints. 2017 5:e2907v1

Type

Empty backbone

Use

Gateway destination vector for protein expression…

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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371 pTol2-fli1ep:MTDest

Plasmid

#73490

Purpose

Tol2 vector with fli1ep element upstream of 6xmyc, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep:6xMyc-attR1-attR2

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pIB169

Plasmid

#90192

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

Depositor

Indranil Biswas

Article

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

His-tag and Strep-tag

Expression

Bacterial

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pUBN-paGFP-Dest

Plasmid

#105108

Purpose

fluorescent tagging in stably transformed plants

Depositor

Christopher Grefen

Article

Blatt et al Methods Mol Biol. 2014;1062:487-507. doi

Type

Empty backbone

Tags

paGFP

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCJF604 - pDESTR4-R3(ttTi4391, I)

Plasmid

#34861

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi4391 targeting

Use

Worm targeting

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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343 pTol2-fli1epDest

Plasmid

#73486

Purpose

Tol2 vector with chimeric endothelial enhancer/promoter upstream of attR1/R2 cassette and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pBsaGSCYP101A1

Plasmid

#108206

Purpose

CYP101A1

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

CYP101A1

Expression

Bacterial

Promoter

lac promoter

Available Since

April 15, 2018

Availability

Academic Institutions and Nonprofits only

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pZa-tetO-csgAW-CmR

Plasmid

#98937

Purpose

Conductive Curli Protein csgAW

Depositor

Urartu Seker

Article

Kalyoncu et al RSC Adv. 2017, Issue 52. doi: 10.1039/C7

Insert

csgAW

Tags

WWRKWKEWDDW

Expression

Bacterial

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pZa-tetO-csgAY-CmR

Plasmid

#98938

Purpose

Conductive Curli Protein csgAY

Depositor

Urartu Seker

Article

Kalyoncu et al RSC Adv. 2017, Issue 52. doi: 10.1039/C7

Insert

csgAY

Tags

YYRKYKEYDDY

Expression

Bacterial

Promoter

tetO

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pJBL2148

Plasmid

#71208

Purpose

pT181 Sense Expression Plasmid

Depositor

Julius Lucks

Article

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

pT181 Sense

Use

Synthetic Biology

Promoter

BBa_J23119

Available Since

Jan. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pWT050a

Plasmid

#107899

Purpose

W2.4-2 plasmid. Contains PLacO-sgRNA1, PRha-sgRNA4 and is part of CAMERA 2.4

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PLacO-sgRNA1, PRha-sgRNA4

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pKR2.40

Plasmid

#26952

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

pie-1promoter-GFP- gateway destination cassette B -pie-1 3’

Tags

GFP

Expression

Worm

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pCH119

Plasmid

#65954

Purpose

Receptor integration for two-strain system

Depositor

Matthew Bennett

Article

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Inserts

RhlR (rhlR )
CinR

Promoter

pTrc*

Available Since

Oct. 6, 2015

Availability

Academic Institutions and Nonprofits only

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MP-Q6

Plasmid

#69676

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA ugi APOBEC1

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHH02_15UP436

Plasmid

#120893

Purpose

Selection of ECF for controller implementation

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

Insert

Pecf15_up436 (-60 to +20)

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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