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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,364 results for: ina

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Showing: 11361 - 11380 of 25364 results

  4. pCMV-hAR-L26A,F27A

    Plasmid
    #89084
    Purpose
    mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif
    Depositor
    Elizabeth Wilson
    Publication
    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hAR FxxLF mutant (AR Human)
    Expression
    Mammalian
    Mutation
    hAR-L26A,F27A
    Promoter
    CMV
    Available since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only

  5. Halo-Tev-Akt3 WT-2X Flag

    Plasmid
    #87607
    Purpose
    expresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of human Akt3 protein
    Depositor
    Yimon Aye
    Insert
    Akt3 isoform 2 (AKT3 Human)
    Use
    Zebrafish (danio rerio) expression
    Tags
    2 tandem flag tags and His-Halo-Tev
    Expression
    Mammalian
    Mutation
    Akt3 isoform 2
    Promoter
    SP6
    Available since
    March 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87607-plasmid-map-sequence-id-197919

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  6. pGEX-2T-FRB-W2027F

    Plasmid
    #73341
    Purpose
    Point mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTOR
    Depositor
    Jie Chen
    Publication
    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72
    Insert
    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    Tags
    GST-tagged
    Expression
    Bacterial
    Mutation
    FRB domain only aa2015-2114; W2027F
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. Sema4c-AP-His

    Plasmid
    #72030
    Purpose
    Expresses the extracellular region of the Sema4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Publication
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sema4c (Sema4c Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72030-plasmid-map-sequence-id-131216

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  8. Sema3a(L, del3)-AP-His

    Plasmid
    #72010
    Purpose
    Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Publication
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sema3a (Sema3a Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72010-plasmid-map-sequence-id-135850

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  9. Sema3a(L, del13)-AP-His

    Plasmid
    #72011
    Purpose
    Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 13), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Publication
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sema3a (Sema3a Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    72011-plasmid-map-sequence-id-131197

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  10. pDual-eGFP(Stop66)

    Plasmid
    #63218
    Purpose
    Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Publication
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Stop66)
    Use
    Lentiviral
    Tags
    T7 and his6
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. Tom20-mChF-AIP(TE)

    Plasmid
    #61522
    Purpose
    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TE mutation
    Depositor
    Takanari Inoue
    Publication
    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FK506 binding protein 1A (FKBP1A Human)
    Tags
    AIP with TE mutation (see comments), Tom20, and m…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pIS1 mIL6R

    Plasmid
    #60797
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains IL6R 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Publication
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    IL6R 3'UTR and mutated miR-155 binding site (IL6R Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available since
    Oct. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    60797-plasmid-map-sequence-id-97808

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  13. pIS1 mATP2B1

    Plasmid
    #60789
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains ATP2B1 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Publication
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP2B1 3'UTR and mutated miR-155 binding site (ATP2B1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available since
    Oct. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    60789-plasmid-map-sequence-id-97792

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  16. MSP10-bio

    Plasmid
    #47719
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP10 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP10
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47719-plasmid-map-sequence-id-68458

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  17. ASP-bio

    Plasmid
    #47745
    Purpose
    Expresses enzymatically monobiotinylated full-length ASP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised ASP
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47745-plasmid-map-sequence-id-68757

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  18. RAP1-bio

    Plasmid
    #47794
    Purpose
    Expresses enzymatically monobiotinylated full-length RAP1 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RAP1
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47794-plasmid-map-sequence-id-68800

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  19. pGL3-24p3/LCN2-282kB mut-LUC

    Plasmid
    #46867
    Purpose
    murine 24p3 minimal promoter with NF-kB mutation as a luciferase reporter
    Depositor
    Sarah Gaffen
    Publication
    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    24p3/lipocalin 2 proximal promoter
    Use
    Luciferase
    Mutation
    NF- kB site is mutated (see comments)
    Promoter
    24p3 promoter (inactive)
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 11361 - 11380 of 25364 results