We narrowed to 759 results for: gc

1. hU6-pegRNA-EF-1α-Cre Template

   Plasmid

   #196036

   Purpose

   The template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.

   Depositor

   Tyler Jacks

   Article

   Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 and EF-1-alpha

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. EFS-SpdCas9-Dnmt3A/3L-catΔ

   Plasmid

   #222501

   Purpose

   Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) followed by P2A-mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Insert

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; C706A and R832…

   Promoter

   EFS

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hsyn-flex-Voltron-ST

   Plasmid

   #119036

   Purpose

   Cre-dependant expression of soma-localized Voltron in neurons

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

   Has Service

   AAV1

   Insert

   Voltron-ST
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hsyn

   Available since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLINC-AKAR1(TA)-CAAX

   Plasmid

   #87706

   Purpose

   Negative-control mutant of FLINC-AKAR1-CAAX.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   FLINC-AKAR1(TA)-CAAX
   

   Use

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PKAsub(TA)-TagRFPT-CAAX

   Plasmid

   #87740

   Purpose

   Negative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   PKAsub(TA)-TagRFPT-CAAX
   

   Use

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-U6-sgRNA-BFP-Puro

   Plasmid

   #229013

   Purpose

   Perturb Seq sgRNA vector with PiggyBac backbone

   Depositor

   Gary Hon

   Article

   Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-U6-sgRNA-BFP-Puro

   Plasmid

   #229014

   Purpose

   Perturb Seq lentiviral sgRNA vector

   Depositor

   Gary Hon

   Article

   Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EFS-SpdCas9-Dnmt3A/3L-V3

   Plasmid

   #222500

   Purpose

   Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Insert

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; R887E and E814…

   Promoter

   EFS

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TRE3GV-SpdCas9-Dnmt3A/3L-V1

   Plasmid

   #222503

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A

   Promoter

   TRE3GV and hPGK

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TRE3GV-SpdCas9-Dnmt3A/3L-V2

    Plasmid

    #222505

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A

    Promoter

    TRE3GV and hPGK

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge