We narrowed to 4,301 results for: PRS

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pRSET-QUEEN 7mu I88D

Plasmid

#129357

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu I88D

Expression

Bacterial

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-His-TEV-OTC_d1_Ub

Plasmid

#170729

Purpose

Scaﬀold design for studying ubiquitin interacting proteins by cryo-EM.

Depositor

Shang-Te Danny Hsu

Article

Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

Insert

E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin

Tags

6xHis-TEV

Expression

Bacterial

Mutation

The C-terminus of OTC was truncated by removing o…

Promoter

T7

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-mNeonGreen (pJJF003)

Plasmid

#173459

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsRCK-HsPatL1_1-111_B

Plasmid

#145974

Purpose

Bacterial Expression of HsPatL1_1-111

Depositor

Elisa Izaurralde

Insert

HsPatL1_1-111 (PATL1 Human)

Expression

Bacterial

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG1-ATG7-3'UTR

Plasmid

#166847

Purpose

Expresses Atg1 with ATG7 3'UTR in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Promoter

ATG1 Promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 SEC66-VAC8-GFP

Plasmid

#166840

Purpose

Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8, SEC66 Budding Yeast)

Tags

GFP and SEC66 transmembrane domain

Expression

Yeast

Mutation

SEC66 transmembrane domain

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-G2A-GFP

Plasmid

#166837

Purpose

Expresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2Ala

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

G2A

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-tglA(C51)HIB

Plasmid

#134675

Purpose

Expresses His6-TglA(C51), TglH, TglI and TglB in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Inserts

TglA(C51)
TglH
TglI
TglB

Tags

His6

Expression

Bacterial

Mutation

insertion of cysteine 51

Promoter

T7

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-3P-ECM33_intron

Plasmid

#127601

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 3′ end of intron

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5M-ECM33_intron

Plasmid

#127600

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5P-ECM33_intron

Plasmid

#127599

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 5′ end of intron

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-ECM33_intron

Plasmid

#127598

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS-ATG1(mut)(406)

Plasmid

#122069

Purpose

Expressing a mutant ATG1

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

Codon changes without changing the amino acid seq…

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG7p-ATG1(406)

Plasmid

#122071

Purpose

Expressing ATG1

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

The native promoter was replaced by ATG7 promoter…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG9-GFP T119D

Plasmid

#114668

Purpose

Expresses yeast ATG9 (T119D) under the endogenous promoter with a GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Promoter

ATG9

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 N295D/D450G

Plasmid

#87287

Purpose

Encodes Hsh155 N295D D450G for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

N295D & D450G

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294L/H331D

Plasmid

#87282

Purpose

Encodes Hsh155 R294L H331D for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

R294L & H331D

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 N295D/H331D

Plasmid

#87285

Purpose

Encodes Hsh155 N295D H331D for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

N295D & H331D

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 N295D/K335E

Plasmid

#87286

Purpose

Encodes Hsh155 N295D K335E for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

N295D & K335E

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294L/D450G

Plasmid

#87284

Purpose

Encodes Hsh155 R294L D450G for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

R294L & D450G

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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