Addgene

We narrowed to 2,078 results for: 3-Oct

Showing: 1121 - 1140 of 2078 results

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pcDNA-DDX11-Flag-WT

Plasmid

#120727

Purpose

Expression of a Flag-tagged version of DDX11 WT in mammalian cells

Depositor

Francesca Pisani

Article

Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

Insert

DDX11 (DDX11 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAX198

Plasmid

#173042

Purpose

Custom vector for sgRNA library construction

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Insert

HBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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mPapaya1-Farnesyl-5

Plasmid

#56650

Purpose

Localization: Membrane, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Farnesyl (HRAS Human)

Use

Tags

mPapaya1

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya-Calnexin-N-14

Plasmid

#56343

Purpose

Localization: Endoplasmic Reticulum, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Calnexin (CANX Human)

Use

Tags

mPapaya

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya1-Actin-C-18

Plasmid

#56400

Purpose

Localization: Actin, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Actin (ACTB Human)

Use

Tags

mPapaya1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
mPapaya-Farnesyl-5

Plasmid

#56643

Purpose

Localization: Membrane, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Farnesyl (HRAS Human)

Use

Tags

mPapaya

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya-LaminA-C-18

Plasmid

#56397

Purpose

Localization: Nuclear Envelope, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

LaminA (LMNA Human)

Use

Tags

mPapaya

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
mPapaya-Actin-C-18

Plasmid

#56395

Purpose

Localization: Actin, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Actin (ACTB Human)

Use

Tags

mPapaya

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya1-Calnexin-N-14

Plasmid

#56350

Purpose

Localization: Endoplasmic Reticulum, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Calnexin (CANX Human)

Use

Tags

mPapaya1

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR-v1-sgABCC4_3

Plasmid

#217434

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 3 targeting ABCC4 (ABCC4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLV_PGK_3xHA-LTB4R(T308A,S310A)

Plasmid

#208094

Purpose

Mammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations with an N-terminal 3x HA tag.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

3xHA-LTB4R(T308A,S310A) (LTB4R Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T308A, S310A

Promoter

PGK

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC22A1

Plasmid

#132034

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A1 (SLC22A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

Plasmid

#104140

Purpose

Expresses TREAT-5'TOP reporter in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Use

Tags

24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSHS293

Plasmid

#87348

Purpose

D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Use

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/D435C/C574S/E945C

Promoter

T7

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSHS248

Plasmid

#87354

Purpose

S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Use

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S/S867C/N1054C

Promoter

T7

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pnEA-NpB-CtNot2-NpS-CtNot3_X

Plasmid

#147968

Purpose

Bacterial Expression of CtNot2-CtNot33

Depositor

Elisa Izaurralde

Article

Raisch et al J Struct Biol. 2018 Dec;204(3):388-395.

Insert

CtNot2-CtNot33

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvH-CtNot1_925-2193_X

Plasmid

#147972

Purpose

Bacterial Expression of CtNot1_925-2193

Depositor

Elisa Izaurralde

Article

Raisch et al J Struct Biol. 2018 Dec;204(3):388-395.

Insert

CtNot1_925-2193

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA

Plasmid

#104097

Purpose

Expresses TREAT-PK co-localization control in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Use

Tags

24xMS2 stem loops in 3'UTR and 24xPP7 stem l…

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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PB-TRE-OKSM

Plasmid

#204498

Purpose

Expresses Oct4, Klf4, Sox2 and c-Myc by tet-on promoter in mammalian cells

Depositor

Wataru Fujii

Article

Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

Inserts

OCT4 (POU5F1 Human)
KLF4 (KLF4 Human)
SOX2 (SOX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEP4 E02S ET2K

Plasmid

#20927

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; SV40LT and Klf4 (SOX2, POU5F1, KLF4 Human)

Use

Episomal/ebna

Tags

Expression

Mutation

Promoter

Available since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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