We narrowed to 74,860 results for: Rest

1. pGEX 4T-1/MKRN1

   Plasmid

   #78757

   Purpose

   To purify GST-tagged MKRN1 recombinant protein

   Depositor

   Jaewhan Song

   Article

   Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

   Insert

   MKRN1 (MKRN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HA-MKRN1 110-482

   Plasmid

   #78747

   Purpose

   To overexpress MKRN1 110-482 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

   Insert

   MKRN1 (MKRN1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   110-482

   Promoter

   CMV

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-HA-MKRN1 1-280

   Plasmid

   #78750

   Purpose

   To overexpress -MKRN1 1-280 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

   Insert

   MKRN1 (MKRN1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   1-280

   Promoter

   CMV

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBGSA FPR1 N297A

   Plasmid

   #66723

   Purpose

   Constitutive expression of FPR1 N297A in mammalian cells.

   Depositor

   Heini Miettinen

   Article

   Gripentrog et al Cell Signal. 2008 Feb;20(2):424-31. Epub

   Insert

   FPR1 (haplotype 3) (FPR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N297A

   Promoter

   SR-alpha

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-myc-Rem2 S334A

   Plasmid

   #51593

   Purpose

   expresses RNAiR myc-tagged Rem2 S-A mutant

   Depositor

   Suzanne Paradis

   Article

   Ghiretti et al J Neurosci. 2013 Apr 10;33(15):6504-15.

   Insert

   Rem2 (Rem2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Serine 334 to Alanine; also RNAi resistant

   Promoter

   CMV

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pUC:FCP:ShBle:FCP:EGFP

   Plasmid

   #85987

   Purpose

   Expresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.

   Depositor

   Thomas Mock

   Article

   Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

   Inserts

   ShBle
   EGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   FCP

   Available since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N1.ARRDC1

   Plasmid

   #38320

   Depositor

   Quan Lu

   Article

   Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   ARRDC1 (ARRDC1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSIV-D3psi/delta env/delta Vif/delta Vpr

   Plasmid

   #132928

   Purpose

   Production of SIV-VLPs containing Vpx protein

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   Gag, pol, and vpx
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-TetOne-Puro-mitoLbNOX-FLAG

   Plasmid

   #218667

   Purpose

   pLVX-TetOne-Puro-mitoLbNOX-FLAG

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   mitoLbNOX
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG and MTS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-TetOne-Puro-LbNOX-FLAG

    Plasmid

    #218631

    Purpose

    This plasmid contains LbNOX under doxycycline inducible control.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    LbNOX
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti_CamKIIa_GFP

    Plasmid

    #96941

    Purpose

    To label mature excitatory iPSC-derived human neurons

    Depositor

    Oleksandr Shcheglovitov, Ricardo Dolmetsch

    Article

    Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

    Insert

    Green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3b-8XGliBS:EGFP

    Plasmid

    #84602

    Purpose

    Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

    Depositor

    James Chen

    Article

    Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    8X Gli-binding site

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCHC005 (∆RE)

    Plasmid

    #226199

    Purpose

    Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for type I restriction-modification system restriction subunit H16_A0006
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. ∆DUSP6

    Plasmid

    #27977

    Depositor

    Igor Astsaturov

    Article

    Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

    Insert

    DUSP6 (DUSP6 Human)
    

    Use

    Lentiviral

    Tags

    HA and MYC

    Expression

    Mammalian

    Mutation

    C293S: mutation in the phosphatase domain

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

16. SiRES COR1C-GFP

    Plasmid

    #195154

    Purpose

    image COR1C

    Depositor

    Gia Voeltz

    Article

    Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    CORO1C (CORO1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutations to make si RNA resistant

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiCRISPRv2-Beclin1

    Plasmid

    #99574

    Purpose

    Expressed Cas9 with sgRNA targeting Beclin1

    Depositor

    Edward Campbell

    Article

    Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

    Insert

    gRNA targeting Beclin1 (BECN1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXS-IRES-Blast coUGP2

    Plasmid

    #211531

    Purpose

    Expresses codon optimized UGP2

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    UGP2 (UGP2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX459V2.0-HypaCas9-mRuby2

    Plasmid

    #183872

    Purpose

    pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HypaCas9-P2A-mRuby2-T2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-c/o NL4-3 Env delta CT

    Plasmid

    #139285

    Purpose

    HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    codon optimized HIV-1 Env delta cytoplasmic tail
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of cytoplasmic tail after residue 710

    Promoter

    CMV IE

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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