We narrowed to 5,610 results for: SPI

1. pAAV-Ef1a-fDIO-mRuby3-2A-Cre

   Plasmid

   #203844

   Purpose

   Flp-dependent Cre and mRuby3 expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Cre
   

   Use

   AAV

   Tags

   mRuby3-2A

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQCXIP-cathepsinD-YMH

   Plasmid

   #182644

   Purpose

   Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   cathepsinD
   

   Use

   Retroviral

   Tags

   His6-tag, TS site from proCCK, and myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Syn1-fDIO-mScarlet-KASH-2a-Cre

   Plasmid

   #203838

   Purpose

   Flp-dependent Cre and mScarlet-KASH expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Cre
   

   Use

   AAV

   Tags

   mScarlet-KASH-2A

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV_NFL(WT)-FLAG

   Plasmid

   #182654

   Purpose

   Expresses wild-type mouse neurofilament light chain with a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   wild type mouse neurofilament light chain (Nefl Mouse)
   

   Use

   Tags

   FLAG tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SQT1665

   Plasmid

   #78744

   Purpose

   Human expression plasmid for LbCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PDZD7-TwinStrepII

   Plasmid

   #83198

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Article

   Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

   Insert

   PDZD7 (Pdzd7 Mouse)
   

   Use

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4046785

   Plasmid

   #145684

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. S1-Fc

   Plasmid

   #164220

   Purpose

   Expresses soluble, dimeric Fc-His fusion protein for the original SARS-CoV2 Spike S1 region, along with dTomato reporter

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

   Insert

   Spike S1 domain (S SARS-CoV)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Gal4-PGC1 alpha

   Plasmid

   #8892

   Depositor

   Bruce Spiegelman

   Article

   Puigserver et al Cell 1998 Mar 20;92(6):829-39.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   Gal4 DBD

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pHYP-NPC-10H

    Plasmid

    #162789

    Purpose

    SARS-CoV-2 Nucleocapsid Protein expression in E.coli

    Depositor

    Ivan Vorobiev

    Article

    Kolesov et al PeerJ. 2022 Jan 7;10:e12751. doi: 10.771

    Insert

    Nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_FLAG-NFL(WT)

    Plasmid

    #182659

    Purpose

    Expresses wild-type mouse neurofilament light chain with an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    wild type mouse neurofilament light chain (Nefl Mouse)
    

    Use

    Tags

    FLAG tag (DYKDDDDK)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGBW-m4046810

    Plasmid

    #149251

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pENTR4_FLAG/HA_FUS_His6

    Plasmid

    #26365

    Depositor

    Thomas Tuschl

    Article

    Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

    Insert

    FUS (FUS Human)
    

    Use

    Entry vector

    Tags

    FLAG/HA and His6

    Expression

    Mutation

    delta 527 (stop codon)

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

14. CBM9-ID-F

    Plasmid

    #171639

    Purpose

    Expresses SARS-CoV-2 Spike epitope

    Depositor

    Francis Nano

    Article

    McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

    Insert

    SARS-CoV-2 Spike protein (S SARS-CoV-2)
    

    Use

    Tags

    CBM-9

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. CBM9-ID-H1

    Plasmid

    #171709

    Purpose

    Expresses SARS-CoV-2 Spike epitope

    Depositor

    Francis Nano

    Article

    McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

    Insert

    SARS-CoV-2 Spike protein (S SARS-CoV-2)
    

    Use

    Tags

    CBM-9

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pENTR4_FLAG/HA_EWSR1_His6

    Plasmid

    #26364

    Depositor

    Thomas Tuschl

    Article

    Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Entry vector

    Tags

    FLAG/HA and His6

    Expression

    Mutation

    delta 657 (stop codon)

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pB-DEST (JDW 1205)

    Plasmid

    #229821

    Purpose

    A PiggyBac destination vector compatible with gateway cloning system.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGG-PIP31

    Plasmid

    #196769

    Purpose

    Contains RBOHD promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    RESPIRATORY BURST OXIDASE HOMOLOGUE D (RBOHD Mustard Weed)
    

    Use

    Greengate (gg) entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPRISM-QF2-he1.1:YFP

    Plasmid

    #184819

    Purpose

    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QF2-pA-he1.1:YFP
    

    Use

    CRISPR; Backbone for cloning of homology arms (fo…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPRISM-QF2-he1.1:YFP-rln3a-HA

    Plasmid

    #184820

    Purpose

    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    rln3a-HA
    

    Use

    CRISPR; For integration of qf2 by homology-direct…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge