We narrowed to 2,016 results for: dox

1. pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA

   Plasmid

   #113696

   Purpose

   U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA

   Plasmid

   #113695

   Purpose

   U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-NDi-CRISPRi (Gen1)

   Plasmid

   #73497

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB-P2A-mCherry
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tet-off Full length Tau (0N4R, P301L/V337M)

   Plasmid

   #188573

   Purpose

   Dox-repressible expression of the 0N4R Tau isoform

   Depositor

   Franz-Ulrich Hartl

   Article

   Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

   Insert

   0N4R Tau (MAPT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   P301L and V337M

   Promoter

   pTight TRE

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVS1-NDi-CRISPRi (Gen2)

   Plasmid

   #73498

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tet-off TauRD (P301L/V337M)

   Plasmid

   #188572

   Purpose

   Dox-repressible expression of the Tau repeat domain

   Depositor

   Franz-Ulrich Hartl

   Article

   Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

   Insert

   Tau repeat domain (MAPT Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P301L and V337M

   Promoter

   pTight TRE

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57.1-MCU-Flag-TetOFF

   Plasmid

   #185657

   Purpose

   Tet/dox-repressible expression of C-terminus Flag tagged human mitochondrial calcium uniporter

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   Mitochondrial calcium uniporter (MCU Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   pTight TRE

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRE3G-GSX2-IRES2-EBF1

   Plasmid

   #96966

   Purpose

   Dox-inducible expression of Gsx2 and Ebf1 in mammalian cells

   Depositor

   Elena Cattaneo

   Article

   Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

   Insert

   GSX2 and EBF1 (bicistronic insert) (Gsx2, Ebf1 Mouse)
   

   Use

   Tags

   FLAG (on EBF1)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

   Plasmid

   #156432

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAU003 - pTRE3G-Cterm_Nterm_switch_CTCF-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid

    #156430

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Cterm_Nterm_switched

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS-TRE-FHDnd1-Ubc-rtTA-I2G

    Plasmid

    #70076

    Purpose

    2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Silent mutations are introduced to escape from RN…

    Promoter

    Tet responsible promoter

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid

    #156434

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    All zinc fingers mutated

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB-6HIS-TEV-mEGFP,IRES-NGFR

    Plasmid

    #164521

    Purpose

    All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

    Depositor

    Mitchell Guttman

    Article

    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G

    Plasmid

    #70075

    Purpose

    2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Y102A. Silent mutations are introduced to escape …

    Promoter

    Tet responsible promoter

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid

    #156429

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    DELTA1-265

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pInducer20_SLC38A2_N82A

    Plasmid

    #156183

    Purpose

    Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    TRE

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSITEP-shBIRC6-2(3)

    Plasmid

    #202451

    Purpose

    DOX-inducible expression of a short-hairpin RNA targetinging human BIRC6

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    BIRC6 shRNA (BIRC6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSITEP-shBIRC6-2

    Plasmid

    #202449

    Purpose

    DOX-inducible expression of a short-hairpin RNA targetinging human BIRC6

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    BIRC6 shRNA (BIRC6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSAM2_mCherry_Gata6

    Plasmid

    #72694

    Purpose

    Doxycyline regulated expression of Gata6 in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Gata 6 (Gata6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-Doxycycline

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

    Plasmid

    #113691

    Purpose

    U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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