We narrowed to 171,796 results for: addgene

Showing: 11421 - 11440 of 171796 results

Results per page:

Sort results by:

LentiGuide-Puro-NCOA7g2 (BB23)

Plasmid

#139458

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pROS15

Plasmid

#107929

Purpose

nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK_hygro_BirA_CDKN1A

Plasmid

#136538

Purpose

Lentiviral expression vector for an inducible BirA-CDKN1A

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

BirA-CDKN1A (CDKN1A )

Use

Lentiviral; Destinatioin vector for gateway cloni…

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTox-EGFPsite1_NGG-PAM (BPK740)

Plasmid

#181746

Purpose

p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAM

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Insert

ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)

Use

Selection plasmid

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SEPHS2 gRNA (BRDN0001148921)

Plasmid

#77661

Purpose

3rd generation lentiviral gRNA plasmid targeting human SEPHS2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SEPHS2 (SEPHS2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC814 - pAAV-SYN1-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

Plasmid

#63887

Purpose

An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the SYN1 promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized low-affinity GCaMP3(10.19)

Use

AAV

Promoter

human SYN1

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSN25

Plasmid

#100110

Purpose

expresses a chimeric SOX2-repressor fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCitrine-P2A-SOX2 fused to full length HP1 alpha (SOX2, CBX5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PHKA1 gRNA (BRDN0001144977)

Plasmid

#77838

Purpose

3rd generation lentiviral gRNA plasmid targeting human PHKA1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PHKA1 (PHKA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
RIPK1 gRNA (BRDN0001146967)

Plasmid

#76534

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK1 (RIPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
RIPK1 gRNA (BRDN0001149147)

Plasmid

#76535

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK1 (RIPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA BRCA1 C64Y

Plasmid

#139326

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 C64Y using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 C64Y using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA BRCA1 E638K

Plasmid

#139327

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 E638K using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 E638K using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
NEK10 gRNA (BRDN0001162335)

Plasmid

#75636

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK10

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK10 (NEK10 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE-HA-CtIP-siR-N289A-H290A

Plasmid

#82032

Purpose

Plasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Sébastien Britton, Patrick Calsou

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

CtIP (RBBP8 Human)

Tags

HA

Expression

Mammalian

Mutation

Endonuclease-dead mutant: N289A+H290A CtIP; Mutat…

Promoter

CMV-tet

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pROS14

Plasmid

#107928

Purpose

LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
non-targeting control gRNA (BRDN0001147780)

Plasmid

#80226

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
OXSM_pLX307

Plasmid

#98357

Purpose

Lentiviral expression of OXSM

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

OXSM (OXSM Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV hSyn1 CaRhAC T2A tDimer

Plasmid

#101722

Purpose

- humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

Depositor

Thomas Oertner

Article

Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

Inserts

CatRhAC
red fluorescent protein

Use

AAV

Mutation

E497K,C569D

Promoter

human Synapsin1 promotor and ribosomal skip seque…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
JA740

Plasmid

#49939

Purpose

Expresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in EGFP for use as a control

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

Tet1CD GFP (TET1 Human)

Use

TALEN

Promoter

EF1a

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pMXs-ms-Klf1

Plasmid

#50785

Purpose

retroviral expression of mouse Klf1

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Klf1 (Klf1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge