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pcDNA5 FRT TO myc Separase delta 55/S1126A

Plasmid

#59825

Purpose

Allows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

delta 55/S1126A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal V435R

Plasmid

#47350

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

V435R

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN L74E

Plasmid

#47355

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, L74E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pHH0103_LCK-1/1

Plasmid

#91332

Purpose

Protein expression and purification of human SH3 domain construct LCK-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LCK-1/1 (LCK Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal L150E

Plasmid

#47346

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

L150E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pFastBac-hDcr-K70A.

Plasmid

#89145

Purpose

Expression of helicase mutant of human Dicer in Sf9 cells

Depositor

Jennifer Doudna

Article

MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

Insert

hDicer (DICER1 Human)

Tags

His6-tev

Expression

Insect

Mutation

K70A

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJB10

Plasmid

#19507

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB10 (DNAJB2 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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HLH Bmal Pas 1-465-VenC

Plasmid

#47364

Purpose

Bmal fragment cloned with Venus tag and used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Hmga2 ATG m7

Plasmid

#52035

Purpose

encodes mutant HMGA2

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Expression

Mammalian

Mutation

all seven let-7 sites in the 3′ UTR mutated (‘m7’)

Promoter

CMV

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HIS DNAJB10

Plasmid

#19560

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB10 (DNAJB2 Mouse)

Tags

His

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJB10

Plasmid

#19479

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB10 (DNAJB2 Mouse)

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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HA-BRPF1

Plasmid

#250445

Purpose

HA-tagged BRPF1 expression in mammalian cells

Depositor

Xiang-Jiao Yang

Article

Ullah et al Mol Cell Biol. 2008 Nov;28(22):6828-43.

Inserts

BRPF1 (BRPF1 Human)
BRPF1

Tags

HA tag at the N-terminal

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pFlagTurboNTD(C1)-RepoMan

Plasmid

#253073

Purpose

Expression of FlagTurboNTD-RepoMan

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

RepoMan (CDCA2 Human)

Tags

FlagTurboNTD

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pFlagTurboNTD(N3)-MYPT1

Plasmid

#253072

Purpose

Expression of MYPT1-FlagTurboNTD

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

ASPP2 (PPP1R12A Human)

Tags

FlagTurboNTD

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pFlagTurboNTD(C1)-HP1gamma

Plasmid

#253074

Purpose

Expression of FlagTurboNTD-HP1gamma

Depositor

Laura Trinkle-Mulcahy

Article

Rajkumar et al iScience. 2026 Feb 28;29(4):115195. doi:

Insert

HP1gamma (CBX3 Human)

Tags

FlagTurboNTD

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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huCofilin WT SSR

Plasmid

#252616

Purpose

Expresses human wild-type cofilin (shRNA-resistant)

Depositor

James Bamburg

Article

Wiggan et al Dev Cell. 2012 Mar 13;22(3):530-43. doi:

Insert

cofilin 1 (CFL1 Human)

Expression

Mammalian

Mutation

silent mutations at nt 67 to 75 in which TCTTCAAC…

Promoter

CMV

Available Since

March 5, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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SHC003BSDDelGFP-hCCDC32-3xFLAG

Plasmid

#243757

Purpose

Express human CCDC32 in mammalian cells with 3xFLAG tag at C terminus.

Depositor

Jingshi Shen

Article

Wan et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Coiled-coil domain-containing protein 32 (CCDC32 Human)

Use

Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pLJC5-HK2-3xFLAG

Plasmid

#239246

Purpose

HK2 lentiviral overexpression vector

Depositor

Jason Cantor

Article

Huggler et al Nat Metab. 2026 Jan 16. doi: 10.1038/s42

Insert

HK2 (HK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert contains the set of silent mutations descr…

Promoter

Ubc

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLJC5-3xMyc-eGFP-OMP25

Plasmid

#239248

Purpose

3xMyc-eGFP-OMP25 lentiviral overexpression vector

Depositor

Jason Cantor

Article

Huggler et al Nat Metab. 2026 Jan 16. doi: 10.1038/s42

Insert

3xMyc-eGFP-OMP25 (SYNJ2BP Human)

Use

Lentiviral

Tags

3xMyc

Expression

Mammalian

Promoter

Ubc

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLJC6-HK2-3xFLAG

Plasmid

#239250

Purpose

HK2 lentiviral overexpression vector

Depositor

Jason Cantor

Article

Huggler et al Nat Metab. 2026 Jan 16. doi: 10.1038/s42

Insert

HK2 (HK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert contains the set of silent mutations descr…

Promoter

Ubc

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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