We narrowed to 18,986 results for: MUT

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pDRF1-GW AKAR3-EV-NR

Plasmid

#182534

Purpose

Expresses the non responsive PKA FRET sensor AKAR3-EV (T506A) in budding yeast

Depositor

Bas Teusink

Article

Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

Insert

AKAR3-EV-NR

Expression

Yeast

Mutation

T506A mutation

Promoter

PMA1

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEB1-Venus SX

Plasmid

#103990

Purpose

Plasmid encoding Venus SX

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Venus SX

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-mVenus JBC

Plasmid

#103987

Purpose

Plasmid encoding mVenus JBC

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus JBC

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AagRNA

Plasmid

#121953

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b tracrRNA/crRNA duplex

Expression

Mammalian

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pReporter_11

Plasmid

#60571

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_11

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 RNAiR2

Plasmid

#51585

Purpose

expresses RNAi-resistant (RNAiR) myc-tagged mouse Rem2 (long version)

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

silent mutations at shRNA 1 and 2 recognition sit…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBP-TL4

Plasmid

#72988

Purpose

RBS_H5

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

H5 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL8

Plasmid

#72992

Purpose

RBS_L1

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L1 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-L1U1H09

Plasmid

#73008

Purpose

L1U1H09 terminator

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L1U1H09 terminator

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-PTEN-H123Y

Plasmid

#28299

Depositor

Maria-Magdalena Georgescu

Article

Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

Insert

PTEN (PTEN Human)

Tags

FLAG

Expression

Mammalian

Mutation

Phosphatase Inactive Mutant His123Tyr

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pTK64_GFP-NuMA-Bonsai

Plasmid

#46351

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

NuMA deleted 414-1549 (NUMA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acid 414-1549; L1589F mutation in N…

Promoter

pBABE

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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CyclinB-GFP

Plasmid

#54465

Purpose

CyclinB-GFP in pIVT vector for in vitro transcription

Depositor

Michael Lampson

Article

Davydenko et al J Cell Biol. 2013 Jul 22;202(2):221-9. d

Insert

CyclinB

Tags

GFP

Expression

Mammalian

Mutation

K67E and I150T mutations compared to GenBank refe…

Available Since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only
pSARSF505

Plasmid

#46832

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

GFPN-NpuDnaE_intein-GFP_C

Tags

GFP_C and H6-GFP_N

Expression

Bacterial

Mutation

stemmer GFP with superfolder mutations S30R and Y…

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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WDR5

Plasmid

#36900

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

WDR5 (WDR5 Human)

Tags

His tag with TEV cleavage

Expression

Bacterial

Mutation

mutation version of plasmid #60121 3UR4

Available Since

Aug. 8, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3-FRB-LplA(W37I)

Plasmid

#34840

Depositor

Alice Ting

Article

Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

Insert

FRB-LplA(W37I)

Tags

FLAG (internal fusion), HA, and His Tag

Expression

Mammalian

Mutation

LplA has Trp37 to Val mutation

Promoter

CMV

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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IZ1181

Plasmid

#21049

Depositor

Tadahide Izumi

Article

Busso et al Oncogene. 2009 Apr 2. 28(13):1616-25.

Insert

human MDM2 (MDM2 Human)

Tags

no tag

Expression

Mammalian

Mutation

no mutation. This is the wild-type human MDM2.

Available Since

July 9, 2009

Availability

Academic Institutions and Nonprofits only
pReporter_10

Plasmid

#60570

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_10

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM24 shRescue

Plasmid

#28139

Depositor

Michelle Barton

Article

Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

Insert

TRIM24 (TRIM24 Human)

Tags

FLAGX2

Expression

Mammalian

Mutation

A489V; aa 308 and 309 mutated for shRNA rescue

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
MBP-TEV-VMA Model vector

Plasmid

#86590

Purpose

This can be used to make MBP-TEV-Cterm with SapI and PstI; and can also make N-term-VMA with NdeI and SapI

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

VMA intein

Tags

His6-MBP

Expression

Bacterial

Mutation

Original SapI site from pMal was mutated out

Promoter

Tac

Available Since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS_2paCRY2(D387A)

Plasmid

#129652

Purpose

2P-activatable CRY2 with the FAD-disrupting mutation

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2(D387A)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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