We narrowed to 19,008 results for: REV

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pBBK71 pHomL-RPL18Bp-ARC35-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179055

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ARC35-Link-Scarlet(rfp), a marker for the actin cytoskeleton

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ARC35-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK69 pHomL-RPL18Bp-SPC42-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179053

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Scarlet(rfp), a marker for the spindle pole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SPC42-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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ANLN A1.2 gRNA

Plasmid

#90530

Purpose

3rd generation lentiviral gRNA plasmid targeting human ANLN

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ANLN (Guide Designation A1.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-AtAFB2-mTagBFP2

Plasmid

#179889

Purpose

Expresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

AtAFB2 (AFB2 Mustard Weed, Synthetic)

Use

Lentiviral

Tags

mTagBFP2

Expression

Bacterial and Mammalian

Promoter

EF1

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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Cntn4.2-Fc-His

Plasmid

#72068

Purpose

Expresses the entire Contactin 2, isoform 2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn4.2 (Cntn4 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgITGB4-1

Plasmid

#121535

Purpose

sgITGB4-1 sequence: GAGGCGCAGTCCTTATCCACA. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

Insert

sgITGB4-1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA GAL4 CREB 1-341 M1

Plasmid

#22973

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

Insert

CREB (Creb1 GAL4-fusion, Rat)

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine of CREB

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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Sema3c(S)-AP-His

Plasmid

#72016

Purpose

Expresses the Sema3C protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pBBK49 pHomL-RPL18Bp-Neon(gfp)-Link-TUB1-SSA1t-HomR (AmpR)

Plasmid

#179033

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-TUB1, a marker for the microtubules

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-TUB1

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-FSF1

Plasmid

#110641

Purpose

Retroviral expression of flag-tagged yeast FSF1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

FSF1 (FSF1 Budding Yeast)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pX330_rActb sgRNA / hSpCas9

Plasmid

#172833

Purpose

Mammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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PPP1CB C3.5 gRNA

Plasmid

#90845

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP1CB

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP1CB (Guide Designation C3.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLV Tmem53-V5

Plasmid

#175142

Purpose

Lentiviral expression of mouse Tmem53-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem53 (Tmem53 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pX330_Tuba1b sgRNA / hSpCas9

Plasmid

#172835

Purpose

Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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TRIP13 H3.2 gRNA

Plasmid

#90920

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIP13

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TRIP13 (Guide Designation H3.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAA3

Plasmid

#154163

Purpose

Firefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Mutation

20 wt NF-κB sites were replaced with Rel1A-specif…

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-At_PRORP1-His

Plasmid

#67869

Purpose

bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

Depositor

Walter Rossmanith

Article

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP1 (PRORP1 Mustard Weed)

Tags

His

Expression

Bacterial

Mutation

Residues 36-572 (see comments)

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-Scara3-IRES-BFP

Plasmid

#117265

Purpose

Retroviral overexpression of Scara3

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Scara3 (Scara3 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

LTR

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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AURKA A2.1 gRNA

Plasmid

#90541

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKA

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

AURKA (Guide Designation A2.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCaSpeR4 cyto Grx1-roGFP2

Plasmid

#64999

Purpose

Expression of cytosolic Grx1-roGFP2 under the alphaTub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP2

Expression

Insect

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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