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pAXY0018

Plasmid

#205587

Purpose

p3flag_HygR(1-52)-NpuDnaE(N)

Depositor

Xiaohan Yang

Publication

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

HygR-NpuDnaE(N)_homo-codon-OP

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV IE94

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB511-TP-UnaG-FLAG

Plasmid

#203479

Purpose

For Agrobacterium transformation. A plastid transit peptide fused UnaG-FLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Publication

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG-FLAG

Expression

Bacterial and Plant

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-2xmNeon

Plasmid

#178462

Purpose

A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Publication

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen dimer

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD_TatABmCherryC

Plasmid

#178545

Purpose

pTatABC with an mCherry fluorescent protein tag at the end of TatB.

Depositor

Siegfried Musser

Publication

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatBmCherry, TatC

Tags

mCherry fused to TatB

Expression

Bacterial

Promoter

araC

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-PIP5K1BD266K

Plasmid

#202723

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1B(D266K) (PIP5K1B Human)

Expression

Mammalian

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-Linker

Plasmid

#192305

Purpose

Gateway entry vector for cloning

Depositor

Kevin Janes

Publication

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

Linker

Use

Gateway Cloning

Tags

3xFLAG

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pWzl_CRKL-W160L+W275L

Plasmid

#196021

Purpose

Expresses CRKL W160L + W275L

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Retroviral

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-miR21mutant-SunTag-TREAT

Plasmid

#196933

Purpose

SunTag cassette was added in the coding region in the miR21mutant -IRE-TREAT reporter.

Depositor

Jeffrey Chao

Publication

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-let7-SunTag-TREAT

Plasmid

#196926

Purpose

SunTag cassette was added in the coding region in the let7-IRE-TREAT reporter

Depositor

Jeffrey Chao

Publication

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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IRE-let7R-SunTag-TREAT

Plasmid

#196927

Purpose

SunTag cassette was added in the coding region in the let7R-IRE-TREAT reporter.

Depositor

Jeffrey Chao

Publication

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Publication

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Expression

Mammalian

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_CoA-Snifit(G41S)

Plasmid

#197940

Purpose

Expression of CoA-Snifit in bacterial cells

Depositor

Kai Johnsson

Publication

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Bacterial

Mutation

PanK=PanK-G41S, D127A,L277W,F252Y

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_CoA-Snifit(V97T)

Plasmid

#197941

Purpose

Expression of CoA-Snifit in bacterial cells

Depositor

Kai Johnsson

Publication

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Bacterial

Mutation

PanK=PanK-V97T, D127A,L277W,F252Y

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3_CRKL-silent-V5

Plasmid

#196015

Purpose

Expresses CRKL-silent, V5 tagged

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Lentiviral

Expression

Mammalian

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3_CRKL-W160L-V5

Plasmid

#196017

Purpose

Expresses CRKL W160L, V5 tagged

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Lentiviral

Expression

Mammalian

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pWzl_CRKL-W160L+Y207F

Plasmid

#196020

Purpose

Expresses Expresses CRKL W160L + Y207F

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Retroviral

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLX317 TFAP4

Plasmid

#193616

Purpose

Expresses TFAP4

Depositor

William Hahn

Publication

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

TFAP4 (TFAP4 Human)

Use

Lentiviral

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ

Plasmid

#192399

Purpose

Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-Rluc-FRT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZ

Plasmid

#192398

Purpose

On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-Rluc-FRT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ

Plasmid

#192404

Purpose

On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

Depositor

Ryan Lister

Publication

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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