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pVC2_C_07_VNP1

Plasmid

#250058

Purpose

NT-CRISPR plasmid (pST_116) with gRNA targetting VNP1

Depositor

Georg Fritz

Publication

Faber et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

NT-CRISPR plasmid (pST_116) with gRNA targetting VNP1

Use

Synthetic Biology

Available since

Oct. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pWN_U6-MCS-miniGag

Plasmid

#261578

Purpose

miniEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter). Includes BsmBI sites for cloning in new sgRNA.

Depositor

Jennifer Doudna

Publication

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag

Expression

Mammalian

Available since

Sept. 16, 2026

Availability

Academic Institutions and Nonprofits only

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Or4-T2A-mCD8:GFP-3XP3-dsRed

Plasmid

#219789

Purpose

Drives CD8:GFP in cells expressing A. aegypti olfactory receptor Or4

Depositor

Carolyn McBride

Publication

David et al Nat Commun. 2026 Aug 15;17(1):9828. doi:

Insert

T2A-cd8GFP-3XP3dsRed

Expression

Insect

Available since

Aug. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLEX-CD63-Intein-Cre

Plasmid

#247722

Purpose

Expresses EV-Sorting domain CD63 and Cre recombinase, separated by self-cleaving intein

Depositor

Samir El Andaloussi

Publication

Liang et al Nat Commun. 2025 Apr 29;16(1):4028. doi:

Insert

CD63-Intein-Cre

Expression

Mammalian

Promoter

CAG

Available since

Aug. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pIVT-desNb_EGFP-BleoR-mRuby2

Plasmid

#252240

Purpose

Template for IVTR of destabilized antibody against EGFP or EBFP with PuroR-mRuby2 as effector

Depositor

Kate Galloway

Insert

T7-desNb-EGFP-BleoR-mRuby2

Use

Synthetic Biology

Expression

Mammalian

Mutation

N/A

Promoter

T7

Available since

July 10, 2026

Availability

Academic Institutions and Nonprofits only

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CLYBL_CAG-TALEN-L1

Plasmid

#252226

Purpose

TALEN-expressing construct to target CLYBL (left)

Depositor

Kate Galloway

Insert

TALEN

Use

Synthetic Biology

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

July 10, 2026

Availability

Academic Institutions and Nonprofits only

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CLYBL_CAG-TALEN-R1

Plasmid

#252227

Purpose

TALEN-expressing construct to target CLYBL (right)

Depositor

Kate Galloway

Insert

TALEN

Use

Synthetic Biology

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

July 10, 2026

Availability

Academic Institutions and Nonprofits only

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pVC2_C_03_fliR

Plasmid

#250054

Purpose

NT-CRISPR plasmid (pST_116) with gRNA targetting vps

Depositor

Georg Fritz

Publication

Faber et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

NT-CRISPR plasmid (pST_116) with gRNA targetting vps

Use

Synthetic Biology

Available since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pVC2_C_01_araA

Plasmid

#250052

Purpose

NT-CRISPR plasmid (pST_116) with gRNA targetting phaBAC

Depositor

Georg Fritz

Publication

Faber et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

NT-CRISPR plasmid (pST_116) with gRNA targetting phaBAC

Use

Synthetic Biology

Available since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pVC2_C_08_vps

Plasmid

#250059

Purpose

NT-CRISPR plasmid (pST_116) with gRNA targetting mScarlet

Depositor

Georg Fritz

Publication

Faber et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

NT-CRISPR plasmid (pST_116) with gRNA targetting mScarlet

Use

Synthetic Biology

Available since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pJB035 pAAV CMV LumiScarlet

Plasmid

#252770

Purpose

An AAV vector that expresses CMV driven LumiScarlet

Depositor

Joshua Leonard

Publication

Boucher et al J Extracell Vesicles. 2026 Apr;15(4):e70

Insert

LumiScarlet

Use

AAV

Promoter

CMV

Available since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pDD1780 pCS2-ACPp

Plasmid

#251803

Purpose

Potyviral protease specific against heptapeptide sequence: ESVSLQS

Depositor

Dave Dingal

Publication

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

ACPp

Expression

Mammalian

Promoter

CMV IE94

Available since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pDD0929 pCS2-LMoVp

Plasmid

#251795

Purpose

Potyviral protease specific against heptapeptide sequence: EIVAFQA

Depositor

Dave Dingal

Publication

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

LMoVp

Expression

Mammalian

Promoter

CMV IE94

Available since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pDD0316 pCS2-TEVp

Plasmid

#251773

Purpose

Potyviral protease specific against heptapeptide sequence: ENLYFQS

Depositor

Dave Dingal

Publication

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

TEVp

Expression

Mammalian

Mutation

S219V

Promoter

CMV IE94

Available since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx2_WT_GFP (ABE8e_L34W_nCas9_WT)

Plasmid

#251549

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the L34W mutation in the deaminase domain

Depositor

George Church

Publication

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_L34W-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx2 L34W

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx4_WT_GFP (ABE8e_V28C_M151E_nCas9_WT)

Plasmid

#251551

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the V28C and M151E mutations in the deaminase domain

Depositor

George Church

Publication

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C_M151E-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx4 V28C_M151E

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx3_WT_GFP (ABE8e_M151E_nCas9_WT)

Plasmid

#251550

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the M151E mutation in the deaminase domain

Depositor

George Church

Publication

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_M151E-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx3 M151E

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pHK408

Plasmid

#235458

Purpose

AND gate receiver with bs for sgRNA2 and sgRNA3

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

AND gate

Use

Synthetic Biology

Available since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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eOYW023

Plasmid

#238404

Purpose

Lux constitutive Golden Gate construct

Depositor

Richard Murray

Publication

Larsson et al ACS Synth Biol. 2025 Oct 17;14(10):4122-

Insert

UNS1-P2m-U3d-lux-t0-UNSX

Expression

Bacterial

Available since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA032

Plasmid

#240781

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Publication

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_high - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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