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PPP2R5D B12.5 gRNA

Plasmid

#90854

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP2R5D

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP2R5D (Guide Designation B12.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSH6 E7.3 gRNA

Plasmid

#90772

Purpose

3rd generation lentiviral gRNA plasmid targeting human MSH6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MSH6 (Guide Designation E7.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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MSH6 E8.3 gRNA

Plasmid

#90773

Purpose

3rd generation lentiviral gRNA plasmid targeting human MSH6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MSH6 (Guide Designation E8.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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MASTL F3.1 gRNA

Plasmid

#90752

Purpose

3rd generation lentiviral gRNA plasmid targeting human MASTL

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MASTL (Guide Designation F3.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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Sema4a-AP-His

Plasmid

#72028

Purpose

Expresses the extracellular region of the Sema4A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4a (Sema4a Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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PSMD14 E5.4 gRNA

Plasmid

#90858

Purpose

3rd generation lentiviral gRNA plasmid targeting human PSMD14

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PSMD14 (Guide Designation E5.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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H2AFX B3.3 gRNA

Plasmid

#90702

Purpose

3rd generation lentiviral gRNA plasmid targeting human H2AFX

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

H2AFX (Guide Designation B3.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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H2AFX B4.3 gRNA

Plasmid

#90703

Purpose

3rd generation lentiviral gRNA plasmid targeting human H2AFX

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

H2AFX (Guide Designation B4.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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Ntn5.z-Fc-His

Plasmid

#72109

Purpose

Expresses the entire Netrin 5, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn5 (Ntn5 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Ntn5.y-AP-His

Plasmid

#71982

Purpose

Expresses the entire Netrin 5, isoform y protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn5.y (Ntn5 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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Ntn5.z-AP-His

Plasmid

#71983

Purpose

Expresses the entire Netrin 5, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn5.z (Ntn5 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pRS316-VTC4

Plasmid

#108691

Purpose

VTC4 sequence expressed in yeast plasmid

Depositor

Michael Downey

Article

Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

Insert

Vacuolar Transporter Chaperone 4

Expression

Yeast

Promoter

VTC4 (native promoter)

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NOSTRIN-1/1-SV1/2

Plasmid

#91323

Purpose

Protein expression and purification of human SH3 domain construct NOSTRIN-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NOSTRIN-1/1-SV1/2 (NOSTRIN Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgITGB1-3

Plasmid

#121534

Purpose

sgITGB1-3 sequence: GTTCAGTGAATGGGAACAACG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

Insert

sgITGB1-3

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3KBP1-1/3

Plasmid

#91555

Purpose

Protein expression and purification of human SH3 domain construct SH3KBP1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3KBP1-1/3 (SH3KBP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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MISP H9.2 gRNA

Plasmid

#90770

Purpose

3rd generation lentiviral gRNA plasmid targeting human MISP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MISP (Guide Designation H9.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB4 (E563K)

Plasmid

#29532

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed glutamic acid 563 to lysine

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only
pCDF11-SFPQ(369-598)

Plasmid

#135438

Purpose

Expresses SFPQ 369-598 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF2-3/3

Plasmid

#91281

Purpose

Protein expression and purification of human SH3 domain construct SH3RF2-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF2-3/3 (SH3RF2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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CSNK1A1 H2.4 gRNA

Plasmid

#90641

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1A1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CSNK1A1 (Guide Designation H2.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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