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CSNK1A1 H1.4 gRNA

Plasmid

#90640

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1A1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CSNK1A1 (Guide Designation H1.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-CCCAP

Plasmid

#136847

Purpose

Mammalian expression of the centrosomal protein CCCAP N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-CCCAP (SDCCAG8 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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mCerulean3-EB3-7

Plasmid

#55416

Purpose

Localization: MT End Binding Protein, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

EB3 (MAPRE3 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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FBXO5 D3.4 gRNA

Plasmid

#90686

Purpose

3rd generation lentiviral gRNA plasmid targeting human FBXO5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FBXO5 (Guide Designation D3.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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MAPRE1 F9.3 gRNA

Plasmid

#90746

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPRE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAPRE1 (Guide Designation F9.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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ZW10 H10.3 gRNA

Plasmid

#90943

Purpose

3rd generation lentiviral gRNA plasmid targeting human ZW10

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ZW10 (Guide Designation H10.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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SKA1 H3.1 gRNA

Plasmid

#90888

Purpose

3rd generation lentiviral gRNA plasmid targeting human SKA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SKA1 (Guide Designation H3.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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Sema6d.5-AP-His

Plasmid

#72045

Purpose

Expresses the extracellular region of the Sema6D, isoform 5 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.5 (Sema6d Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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kank3_pJet1.2

Plasmid

#121981

Purpose

for generating kank3 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank3 (kank3 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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kank4_pJet1.2

Plasmid

#121982

Purpose

for generating kank4 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank4 (kank4 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pECE M2-SNX17 S437A

Plasmid

#69812

Purpose

Expresses M2-SNX17 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Sorting nexin-17 (SNX17 Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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ACTL6A C8.3 gRNA

Plasmid

#90521

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACTL6A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ACTL6A (Guide Designation C8.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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ACTL6A C7.3 gRNA

Plasmid

#90520

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACTL6A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ACTL6A (Guide Designation C7.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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MAD2L1 E11.1 gRNA

Plasmid

#90742

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAD2L1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAD2L1 (Guide Designation E11.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pAP2

Plasmid

#41755

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc E. coli, Fly)

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C45S C338S K349C C451S

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
CBX1 A3.5 gRNA

Plasmid

#90566

Purpose

3rd generation lentiviral gRNA plasmid targeting human CBX1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CBX1 (Guide Designation A3.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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CBX1 A4.5 gRNA

Plasmid

#90567

Purpose

3rd generation lentiviral gRNA plasmid targeting human CBX1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CBX1 (Guide Designation A4.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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CBX5 A2.5 gRNA

Plasmid

#90571

Purpose

3rd generation lentiviral gRNA plasmid targeting human CBX5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CBX5 (Guide Designation A2.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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CBX5 A1.5 gRNA

Plasmid

#90570

Purpose

3rd generation lentiviral gRNA plasmid targeting human CBX5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CBX5 (Guide Designation A1.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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PBK D1.3 gRNA

Plasmid

#90824

Purpose

3rd generation lentiviral gRNA plasmid targeting human PBK

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PBK (Guide Designation D1.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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