We narrowed to 6,478 results for: nls

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pSmart-EF1alpha-AcrIC9-NLS

Plasmid

#207397

Purpose

Expresses AcrIC9 with NLS in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIC9-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDESTpK7WG2-RPS5Ap-NLS-OleGFP

Plasmid

#193370

Purpose

nTALEN or nTALECD vector assembly, Destination vector for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.

Depositor

Shin-ichi Arimura

Article

Hosoda et al Plant Biotechnol (Tokyo). 2024 Dec 25;41

Type

Empty backbone

Expression

Plant

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E2_pENTR_R4-R3_HSPter-RPS5A-NLS

Plasmid

#193371

Purpose

nTALEN or nTALECD vector assembly, Entry clone for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.

Depositor

Shin-ichi Arimura

Article

Hosoda et al Plant Biotechnol (Tokyo). 2024 Dec 25;41

Type

Empty backbone

Expression

Plant

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT4_short_mCherry2-NLS-PEST_Puro

Plasmid

#213774

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT4_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT4_mCherry2-NLS-PEST_Puro

Plasmid

#213773

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT4_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT3_short_mCherry2-NLS-PEST_Puro

Plasmid

#213772

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT3_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT2_mCherry2-NLS-PEST_Puro

Plasmid

#213769

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT2_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT2_short_mCherry2-NLS-PEST_Puro

Plasmid

#213770

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT2_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT1_mCherry2-NLS-PEST_Puro

Plasmid

#213767

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT1_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT1_short_mCherry2-NLS-PEST_Puro

Plasmid

#213768

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT1_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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eA3Ai-Cas9n-UGI-NLS

Plasmid

#207165

Purpose

Express A3A with an N57G point mutation and an intron in a BE3-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)

Tags

Cas9-UGI--NLS

Expression

Mammalian

Mutation

N57G

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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PET-HypaSpCas9-NLS-6xHis

Plasmid

#207384

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N692A, M694A, Q695A, H698A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-HeFSpCas9-NLS-6xHis

Plasmid

#207391

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HeFSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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PET-evoSpCas9-NLS-6xHis

Plasmid

#207389

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

evoSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

M495V, Y515N, K526E, R661Q

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1a-jAspSnFR3-mRuby3-NLS

Plasmid

#203489

Purpose

Nuclear directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3-NLS

Use

Gateway entry vector

Tags

Nuclear localization signal and mRuby3

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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iB22 PB-zim3-mycNLS-mApple

Plasmid

#204718

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Expression

Mammalian

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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MCS-T2a-GFPnls-ER-Cre-ER

Plasmid

#200104

Purpose

Vector For Twist

Depositor

Prayag Murawala

Type

Empty backbone

Use

Unspecified

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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Twist3cterm-GFPnls-T2A-ER-Cre-ER

Plasmid

#200105

Depositor

Prayag Murawala

Insert

Twist3:cTer-KI-eGFPnls-T2A-ER-Cre-ER

Use

Unspecified

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT IAA17-6FLAG (no NLS)

Plasmid

#193322

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

6FLAG

Expression

Yeast

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-2X(CMV-eBFP2-3XNLS-pA)

Plasmid

#195867

Purpose

to express nuclear localized eBFP2

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

eBFP2

Use

AAV

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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