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WNV NS2B-NS3 protease (K104A - catalytically active, don't self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204794

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available Since

Aug. 29, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pKC31

Plasmid

#226777

Purpose

Expresses Drosophila UNC-104(R562H) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (R562H) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Arginine 562 to Histidine (bris mutation)

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKC52

Plasmid

#226780

Purpose

Expresses Drosophila UNC-104(R348G) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (R348G) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Arginine 348 to Glycine

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKC50

Plasmid

#226778

Purpose

Expresses Drosophila UNC-104(V6M) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (V6M) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Valine 6 to Methionine

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKC28

Plasmid

#226776

Purpose

Expresses Drosophila UNC-104(G618R) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (G618R) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

G618R

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSN539

Plasmid

#226775

Purpose

Expresses Drosophila UNC-104(wild-type) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKC51

Plasmid

#226779

Purpose

Expresses Drosophila UNC-104(A255V) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (A255V) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Alanine 255 to Valine

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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p305N-21

Plasmid

#246304

Purpose

Evaluation of MtU6.6-104 promoter (Pol III promoter) deletion (104 bp) for CRISPR-Cas9 editing of mEGFP in Nicotiana benthamiana and poplar reporter lines

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

MtU6.6-104 promoter

Use

CRISPR

Expression

Plant

Promoter

MtU6.6-104 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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deltaCT S6K1 MSCV puro

Plasmid

#8465

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

delta ct p70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

deletion of 104 c-terminal amino acids

Available Since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
F5A deltaCT S6K1 MSCV puro

Plasmid

#8464

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

f5a delta ctp70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

deletion of 104 c-terminal amino acids mutation …

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pSpliceExpress

Plasmid

#32485

Depositor

Stefan Stamm

Article

Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

Type

Empty backbone

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pDESTsplice

Plasmid

#32484

Depositor

Stefan Stamm

Article

Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

Type

Empty backbone

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA_mouse_Adar2_Tv1

Plasmid

#158110

Purpose

expresses mADAR2 shortest transcript

Depositor

Emanuela Felley-Bosco

Insert

mAdar2 (Adarb1 Mouse)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pVS1

Plasmid

#33143

Depositor

Michael Rosbash

Article

Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

Insert

LacO (256 copies)

Expression

Yeast

Available Since

Dec. 20, 2011

Availability

Academic Institutions and Nonprofits only
pTGV-Express-CAT SMR5916

Plasmid

#28039

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Expression

Bacterial

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pBAC-RT

Plasmid

#16418

Depositor

Craig Strathdee

Article

Stavropoulos et al Genomics. 2001 Feb 15. 72(1):99-104.

Insert

E. coli rpsL gene; tetA(C) gene

Use

Bacmid

Available Since

June 2, 2008

Availability

Academic Institutions and Nonprofits only
pTGV-Tet SMR5970

Plasmid

#28037

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Expression

Bacterial

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pTGV-Kan SMR5938

Plasmid

#28036

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Expression

Bacterial

Available Since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

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pTGV-Express SMR5448

Plasmid

#28031

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Expression

Bacterial

Available Since

May 18, 2011

Availability

Academic Institutions and Nonprofits only
pTGV-Light SMR5452

Plasmid

#28032

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Expression

Bacterial

Available Since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

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