Addgene

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pLXMA_RK2 L2A

Plasmid

#153361

Purpose

Expression vector for plants with low-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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p281 TIR1_HA_flox STOP_mH11

Plasmid

#164856

Purpose

Cre-inducible TIR1 expression vector for mouse H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

OsTIR1

Use

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-UL32

Plasmid

#162664

Purpose

Lentiviral vector for stable expression of UL32 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pME-tdTomato no stop

Plasmid

#82404

Purpose

Gateway-compatible tdTomato fusion reporter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

tdTomato (lacks stop codon)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PalmPalm-ClopHensor

Plasmid

#25940

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

PalmPalm-ClopHensor

Use

Tags

Gap20 and Strep-II

Expression

Mammalian

Mutation

N-term (20AA) of Gap-43

Promoter

Available since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pET11a+CYP119

Plasmid

#66131

Purpose

Expresses the wild type of CYP119

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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p3E-nlsGFP no-pA

Plasmid

#80815

Purpose

nlsGFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-CMV-nlsGFP no pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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p104_gRNA_Sp_CD3e

Plasmid

#213780

Purpose

Expresses CRISPRi gRNA for CD3e promoter and GFP. gRNA driven by mU6.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sp gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBadcrtEBI_13

Plasmid

#63811

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pYES2.1-cerMET8

Plasmid

#107439

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET8 (MET8 Budding Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5myc POSH

Plasmid

#15908

Depositor

Alan Hall

Article

Tapon et al EMBO J. 1998 Mar 2. 17(5):1395-404.

Insert

POSH (Sh3rf1 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCTCON2-Aga1p

Plasmid

#158141

Purpose

Expresses Aga1p in yeast

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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PX853

Plasmid

#62885

Purpose

N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa 2-573)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Mutation

Promoter

CBh

Available since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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BCJJ125

Plasmid

#117515

Purpose

Cas9_3 (with intron) Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:AATG:Cas9_3(intron):GCTT:BsaI

Use

CRISPR

Tags

2xFLAG and NLS

Expression

Plant

Mutation

Promoter

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL11015

Plasmid

#117499

Purpose

FAST-Red selectable marker Golden Gate Level 1 Position 1

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI

Use

Tags

RFP

Expression

Plant

Mutation

Promoter

AtOLE1

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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p273 EF1a_mCherry2_hH11

Plasmid

#164852

Purpose

mCherry2-expression vector for human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

mCherry2

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMLM802

Plasmid

#27203

Depositor

Keith Joung

Article

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pMLM800

Plasmid

#27202

Depositor

Keith Joung

Article

Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

Insert

FokI endonuclease

Use

Zebrafish expression

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

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BCJJ345

Plasmid

#117504

Purpose

YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtYAO

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pOG231

Plasmid

#17736

Depositor

Geoff Wahl

Article

O'Gorman et al Proc Natl Acad Sci U S A. 1997 Dec 23;94

Insert

NLS-Cre recombinase

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only