We narrowed to 3,644 results for: ORF1
-
Plasmid#186765PurposeAn mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C3orf17 sgRNA 2
Plasmid#70653PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C3orf17
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE smFP-V5 donor ORF1
Plasmid#200381PurposeTagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminalDepositorInsertspaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
ExpressionMammalianMutationNAAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE HA-epitope donor ORF1
Plasmid#200372PurposeTagging endogenously expressed proteins with HA epitope, C-terminalDepositorInsertHA epitope
ExpressionMammalianMutationNAAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE V5-epitope donor ORF1
Plasmid#200378PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
ExpressionMammalianMutationNAAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p
Plasmid#202589PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialAvailable SinceJune 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_R261A
Plasmid#202586PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Arginine 261 to AlanineAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOneZeo-ORF18(WT)-3xFLAG
Plasmid#129746PurposeDox inducible expression of KSHV ORF18-3xFLAG in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseLentiviralTags3xFLAGExpressionMammalianPromoterTRE3Gs and Tet inducible promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS
Plasmid#52345PurposeIntegration cassette to create p1-Short-Leu-mKate with p2 ORF10 UCS>mKateDepositorInsertmKate2
UseP1 integration vectorExpressionBacterialMutationchanged V2HPromoterpGKL2 ORF10 UCSAvailable SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23[uORF1/luc2/minP]
Plasmid#226459PurposeMET 5'UTR (uORF1 mutated) upstream FLucDepositorInsertMET 5'UTR uORF1 mutated
ExpressionMammalianAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23[uORF1,2/luc2/minP]
Plasmid#226461PurposeMET 5'UTR (uORF1 and uORF2 mutated) upstream FLucDepositorInsertMET 5'UTR uORF1 uORF2 mutated
ExpressionMammalianAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_K3AK4A
Plasmid#202585PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Lysine 3 to Alanine, changed ORF1p …Available SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 ORF10wu
Plasmid#149332PurposeGateway-compatible Entry vector, with insert of ORF10wu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020DepositorInsertORF10wu (ORF10 SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorMutationMany synonymous changes due to codon optimizationAvailable SinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-SARS-CoV-2 Orf10
Plasmid#158380PurposeMAC-tagged gene expressionDepositorAvailable SinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
ATF4 10: 5’ATF4.uORF1.GFP
Plasmid#21857DepositorInsertATF4 (Atf4 Mouse)
TagsEGFPExpressionMammalianMutationupstream ORF1 (uORF) of ATF4 fused to EGFPAvailable SinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
ZE14 pCS2P+ wnt8 ORF1
Plasmid#17048DepositorInsertwnt8 (wnt8a Zebrafish)
Available SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 ORF10wu_nostop
Plasmid#152986PurposeGateway-compatible Entry vector, with insert of ORF10wu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020DepositorInsertORF10wu
UseGateway-compatible entry vectorMutationMany synonymous changes due to codon optimizationAvailable SinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
ATF4 14: 5’ATF4.uORF1&2AUA.GFP
Plasmid#21861DepositorInsertATF4 (Atf4 Mouse)
TagsEGFPExpressionMammalianMutationAUG codons of uORFs1&2 were converted to AUAAvailable SinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
ATF4 17: 5’ATF4.uORF1stopm1&2.GFP
Plasmid#21863DepositorInsertATF4 (Atf4 Mouse)
TagsEGFPExpressionMammalianMutationStop codons between the colinear uORF1 and uORF2 …Available SinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
ATF4 15: 5’ATF4.uORF1stopm1.GFP
Plasmid#21862DepositorInsertATF4 (Atf4 Mouse)
TagsEGFPExpressionMammalianMutationStop codon for uORF1 converted to UCG (see author…Available SinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only