Addgene

We narrowed to 1,583 results for: abr

Showing: 1141 - 1160 of 1583 results

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pLV[Exp]-Puro-EF-1α>{H2B-RFP}

Plasmid

#201121

Purpose

RFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclear

Depositor

Ori Bar-Nur

Article

Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

Insert

H2B-RFP

Use

Lentiviral

Tags

Expression

Mutation

No

Promoter

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA6.2EmGFP-cDAXX

Plasmid

#169727

Purpose

Mammalian expression construct of GFP tagged cDAXX

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

cDAXX (DAXX Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Comprises residues 495-740

Promoter

CMV

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJEP302-pAAV-CMV-dSaCas9-KRAB-pA

Plasmid

#113677

Purpose

A CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Expression

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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px330 Rosa26 Gu1 (LH416)

Plasmid

#99629

Purpose

Plasmid used to target the wildtype Rosa26 allele with Cas9

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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MCP-3x9aaTAD

Plasmid

#210707

Purpose

This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

N55K in MCP

Promoter

EF1a

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

Plasmid

#113681

Purpose

A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Expression

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B GG

Plasmid

#89451

Purpose

Expression of GFP-tagged human Rab27B GG in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B GG (RAB27B Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

GG (geranyl geranyl site is mutated)

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Dcst1-3xFLAG

Plasmid

#183542

Purpose

Expression vector of mouse DC-STAMP domain containing 1 (Dcst1) tagged with 3xFLAG at C-terminus.

Depositor

Masahito Ikawa

Article

Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

Insert

DC-STAMP domain containing 1 (Dcst1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP303-pAAV-CMV-dSaCas9-VP64-pA

Plasmid

#113678

Purpose

A CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV, CRISPR, and Synthetic Biology

Tags

NLS and VP64

Expression

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-R438E

Plasmid

#91984

Purpose

Expresses FLAG-HA-AGO2 (R438E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

R438E

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-SpdCas9-W3SL

Plasmid

#210708

Purpose

This Plasmid express hSyn promoter driven SpdCas9

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

S. Pyogenes dCas9

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

D10A/H840A

Promoter

hSyn

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-tdTomato-f

Plasmid

#127092

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of farnesylated (f) tdTomato

Depositor

Viviana Gradinaru

Article

Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

Insert

tdTomato-f

Use

AAV

Tags

farnesylation signal from c-Ha-Ras

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-K525E

Plasmid

#91985

Purpose

Expresses FLAG-HA-AGO2 (K525E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

K525E

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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LoxP2-Mb2Tomato-2A (SO240)

Plasmid

#99614

Purpose

To clone gene of interest downstream of LoxP2-Mb2Tomato-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2Tomato

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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LoxP1-Mb2YFP-2A (SO244)

Plasmid

#99613

Purpose

To clone gene of interest downstream of LoxP1-Mb2YFP-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2EYFP

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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LoxP2-H2B-EGFP-V5-2A (SO107)

Plasmid

#99617

Purpose

To clone gene of interest downstream of LoxP2-H2B-EGFP-V5-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

H2B-EGFP-V5

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-D358K

Plasmid

#91983

Purpose

Expresses FLAG-HA-AGO2 (D358K)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D358K

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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LoxP3-HA-H2B-Cerulean-2A (SO104)

Plasmid

#99618

Purpose

To clone gene of interest downstream of LoxP3-HA-H2B-Cerulean-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

HA-H2B-Cerulean

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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natMX-ins7

Plasmid

#195044

Purpose

pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

NatR

Use

Yeast genomic targeting

Tags

tCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…

Expression

Mutation

Promoter

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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LoxP3-Mb2-HA-mTFP1-2A (SO157)

Plasmid

#99615

Purpose

To clone gene of interest downstream of LoxP3-Mb2-HA-mTFP1-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2-HA-mTfp1

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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