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RaCCR1_EYFP_pcDNA3.1

Plasmid

#159122

Purpose

This plasmid encodes RaCCR1 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RaCCR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pIS1-miR-31 site

Plasmid

#25021

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-31 site

Use

Luciferase

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pEPQD0CM0029

Plasmid

#162311

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, strep tag, stop codon

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0284

Plasmid

#162289

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pFCK(1.3)tdimer2W

Plasmid

#27233

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

DsRed2

Use

Lentiviral

Tags

tandem dimer RFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pOZ-N-FH PSMD14

Plasmid

#27500

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

PSMD14 (PSMD14 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

M1V

Available Since

March 1, 2011

Availability

Academic Institutions and Nonprofits only
pTYB1-T4gp46

Plasmid

#228211

Purpose

Expression in BL21(DE3) of T4 SbcC-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

46

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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Gal4-pcVP16-1

Plasmid

#231570

Purpose

Expresses the single component, light-off regulated pcVP16-1 transcriptional regulator

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

Gal4-pcVP16-1

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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Zdk3-VPR

Plasmid

#231572

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

Zdk3-VPR, Ef1a driven

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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cpLOV2-Gal4

Plasmid

#231569

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

cpLOV2-Gal4

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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Zdk3-VP64

Plasmid

#231571

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

Zdk3-VP64

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_Zdk3-VPR

Plasmid

#231573

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

Zdk3-VPR, CMV driven

Expression

Mammalian

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pZZMP132

Plasmid

#231757

Purpose

Mammalian expression of SPO11-EA

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

SPO11-EA (Spo11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

E224A

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-RAD51

Plasmid

#222567

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of RAD51

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

RAD51 (RAD51 Human)

Expression

Mammalian

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-PAX6b

Plasmid

#222577

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of PAX6b

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

PAX6b (PAX6 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAN008

Plasmid

#220049

Purpose

Reporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope (positive control).

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

flag-HA-GFP

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAN012

Plasmid

#220051

Purpose

Reporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide (positive control).

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

GFP-fLuc

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAN053

Plasmid

#220047

Purpose

Mammalian expression of type III-A crRNA from Streptococcus thermophilus within 5`UTR of mCherry transcript

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA-mCherry

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAN102

Plasmid

#220045

Purpose

Mammalian expression of type III-A crRNA and Cas6 crRNA-processing ribonuclease from Streptococcus thermophilus

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA; Cas6

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJTT002

Plasmid

#204493

Purpose

Reporter circuit

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

TagBFP
EYFP
mKate2

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

hEF1a

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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